Literature DB >> 20007281

A rotavirus spike protein conformational intermediate binds lipid bilayers.

Shane D Trask1, Irene S Kim, Stephen C Harrison, Philip R Dormitzer.   

Abstract

During rotavirus entry, a virion penetrates a host cell membrane, sheds its outer capsid proteins, and releases a transcriptionally active subviral particle into the cytoplasm. VP5, the rotavirus protein believed to interact with the membrane bilayer, is a tryptic cleavage product of the outer capsid spike protein, VP4. When a rotavirus particle uncoats, VP5 folds back, in a rearrangement that resembles the fusogenic conformational changes in enveloped-virus fusion proteins. We present direct experimental evidence that this rearrangement leads to membrane binding. VP5 does not associate with liposomes when mounted as part of the trypsin-primed spikes on intact virions, nor does it do so after it has folded back into a stably trimeric, low-energy state. But it does bind liposomes when they are added to virions before uncoating, and VP5 rearrangement is then triggered by addition of EDTA. The presence of liposomes during the rearrangement enhances the otherwise inefficient VP5 conformational change. A VP5 fragment, VP5CT, produced from monomeric recombinant VP4 by successive treatments with chymotrypsin and trypsin, also binds liposomes only when the proteolysis proceeds in their presence. A monoclonal antibody that neutralizes infectivity by blocking a postattachment entry event also blocks VP5 liposome association. We propose that VP5 binds lipid bilayers in an intermediate conformational state, analogous to the extended intermediate conformation of enveloped-virus fusion proteins.

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Year:  2009        PMID: 20007281      PMCID: PMC2812363          DOI: 10.1128/JVI.01682-09

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  28 in total

1.  Assembly of highly infectious rotavirus particles recoated with recombinant outer capsid proteins.

Authors:  Shane D Trask; Philip R Dormitzer
Journal:  J Virol       Date:  2006-09-13       Impact factor: 5.103

2.  Alternative intermolecular contacts underlie the rotavirus VP5* two- to three-fold rearrangement.

Authors:  Joshua D Yoder; Philip R Dormitzer
Journal:  EMBO J       Date:  2006-03-02       Impact factor: 11.598

3.  Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction.

Authors:  Xing Zhang; Ethan Settembre; Chen Xu; Philip R Dormitzer; Richard Bellamy; Stephen C Harrison; Nikolaus Grigorieff
Journal:  Proc Natl Acad Sci U S A       Date:  2008-01-31       Impact factor: 11.205

4.  Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM.

Authors:  James Z Chen; Ethan C Settembre; Scott T Aoki; Xing Zhang; A Richard Bellamy; Philip R Dormitzer; Stephen C Harrison; Nikolaus Grigorieff
Journal:  Proc Natl Acad Sci U S A       Date:  2009-06-01       Impact factor: 11.205

5.  Rotavirus architecture at subnanometer resolution.

Authors:  Zongli Li; Matthew L Baker; Wen Jiang; Mary K Estes; B V Venkataram Prasad
Journal:  J Virol       Date:  2008-11-26       Impact factor: 5.103

6.  High-resolution molecular and antigen structure of the VP8* core of a sialic acid-independent human rotavirus strain.

Authors:  Nilah Monnier; Kyoko Higo-Moriguchi; Zhen-Yu J Sun; B V Venkataram Prasad; Koki Taniguchi; Philip R Dormitzer
Journal:  J Virol       Date:  2006-02       Impact factor: 5.103

7.  Trypsin activation pathway of rotavirus infectivity.

Authors:  C F Arias; P Romero; V Alvarez; S López
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

8.  Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.

Authors:  L Padilla-Noriega; R Werner-Eckert; E R Mackow; M Gorziglia; G Larralde; K Taniguchi; H B Greenberg
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

9.  Antigenicity, immunogenicity and passive protection induced by immunization of mice with baculovirus-expressed VP7 protein from rhesus rotavirus.

Authors:  L Fiore; S J Dunn; B Ridolfi; F M Ruggeri; E R Mackow; H B Greenberg
Journal:  J Gen Virol       Date:  1995-08       Impact factor: 3.891

10.  Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.

Authors:  Scott T Aoki; Ethan C Settembre; Shane D Trask; Harry B Greenberg; Stephen C Harrison; Philip R Dormitzer
Journal:  Science       Date:  2009-06-12       Impact factor: 47.728

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  32 in total

1.  Rhesus rotavirus trafficking during entry into MA104 cells is restricted to the early endosome compartment.

Authors:  Marie Wolf; Emily M Deal; Harry B Greenberg
Journal:  J Virol       Date:  2012-01-25       Impact factor: 5.103

2.  Effect of mutations in VP5 hydrophobic loops on rotavirus cell entry.

Authors:  Irene S Kim; Shane D Trask; Marina Babyonyshev; Philip R Dormitzer; Stephen C Harrison
Journal:  J Virol       Date:  2010-04-07       Impact factor: 5.103

3.  Atomic model of an infectious rotavirus particle.

Authors:  Ethan C Settembre; James Z Chen; Philip R Dormitzer; Nikolaus Grigorieff; Stephen C Harrison
Journal:  EMBO J       Date:  2010-12-14       Impact factor: 11.598

Review 4.  Interactions among capsid proteins orchestrate rotavirus particle functions.

Authors:  Shane D Trask; Kristen M Ogden; John T Patton
Journal:  Curr Opin Virol       Date:  2012-05-16       Impact factor: 7.090

5.  Mutations in the rotavirus spike protein VP4 reduce trypsin sensitivity but not viral spread.

Authors:  Shane D Trask; J Denise Wetzel; Terence S Dermody; John T Patton
Journal:  J Gen Virol       Date:  2013-02-20       Impact factor: 3.891

Review 6.  Structural insights into the coupling of virion assembly and rotavirus replication.

Authors:  Shane D Trask; Sarah M McDonald; John T Patton
Journal:  Nat Rev Microbiol       Date:  2012-01-23       Impact factor: 60.633

7.  Rotaviruses reach late endosomes and require the cation-dependent mannose-6-phosphate receptor and the activity of cathepsin proteases to enter the cell.

Authors:  Marco A Díaz-Salinas; Daniela Silva-Ayala; Susana López; Carlos F Arias
Journal:  J Virol       Date:  2014-02-05       Impact factor: 5.103

8.  Rhesus rotavirus entry into a polarized epithelium is endocytosis dependent and involves sequential VP4 conformational changes.

Authors:  Marie Wolf; Phuoc T Vo; Harry B Greenberg
Journal:  J Virol       Date:  2010-12-29       Impact factor: 5.103

Review 9.  Inflammatory and oxidative stress in rotavirus infection.

Authors:  Carlos A Guerrero; Orlando Acosta
Journal:  World J Virol       Date:  2016-05-12

10.  Determinants of the specificity of rotavirus interactions with the alpha2beta1 integrin.

Authors:  Fiona E Fleming; Kate L Graham; Yoshikazu Takada; Barbara S Coulson
Journal:  J Biol Chem       Date:  2010-12-06       Impact factor: 5.157

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