| Literature DB >> 20006585 |
Madoka Yoshida1, Kyohei Higashi, Lihua Jin, Yoshiki Machi, Takehiro Suzuki, Akiko Masuda, Naoshi Dohmae, Akiko Suganami, Yutaka Tamura, Kazuhiro Nishimura, Toshihiko Toida, Hideyuki Tomitori, Keiko Kashiwagi, Kazuei Igarashi.
Abstract
It is known that the level of protein-conjugated acrolein in plasma is a good marker of chronic renal failure and brain infarction. Thus, studies were carried out to determine which kinds of plasma proteins are conjugated with acrolein. It was found that acrolein was mainly conjugated with albumin. Tandem mass spectrometry analysis demonstrated that Lys-557 and Lys-560, located at the surface of domain III of albumin, were the major sites conjugated with acrolein. This is the first report to identify the amino acid residues in a protein modified by acrolein in vivo. It was found that conjugation of acrolein with albumin contributed to a decrease in the toxicity of acrolein. Copyright 2009 Elsevier Inc. All rights reserved.Entities:
Mesh：
Substances：
Year: 2009 PMID： 20006585 DOI： 10.1016/j.bbrc.2009.12.049
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575