Yanjun Chen1, Wei Liu, Weimin Li, Cheng Gao. 1. Department of Cardiology, the 4th affiliated hospital of Harbin Medical University, Harbin, PR China.
Abstract
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) could differentiate into cardiomyocytes. In this study, we wished to determine whether we could improve ventricular function in a dilated cardiomyopathy (DCM) model in the rabbit by injecting stem cells directly into the ventricular wall. METHODS AND RESULTS: Japanese rabbits were treated with doxorubicin to induce dilated cardiomyopathy. BMSCs were isolated from isogenic adult rabbits and expanded ex vivo. DCM-model animals received an injection directly into the ventricular wall with either BMSCs or saline (sham-operated control; a third control group underwent no operation). Heart function in all three groups was evaluated by echocardiography and cardiac catheterization. Hearts were later excised for histological and immunostaining analysis. Left ventricular function was significantly better in BMSC-transplanted animals than in controls. Cellular staining of BrdU and N-cadherin, indicating the presence of bone-marrow-derived cells, was found in BMSC-transplanted animals but not in control and sham-operated animals. CONCLUSIONS: BMSC transplantation can improve left ventricular function in DCM. Direct injection into the ventricular wall is an effective approach for stem cell delivery. Crown Copyright 2009. Published by Elsevier Inc. All rights reserved.
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) could differentiate into cardiomyocytes. In this study, we wished to determine whether we could improve ventricular function in a dilated cardiomyopathy (DCM) model in the rabbit by injecting stem cells directly into the ventricular wall. METHODS AND RESULTS: Japanese rabbits were treated with doxorubicin to induce dilated cardiomyopathy. BMSCs were isolated from isogenic adult rabbits and expanded ex vivo. DCM-model animals received an injection directly into the ventricular wall with either BMSCs or saline (sham-operated control; a third control group underwent no operation). Heart function in all three groups was evaluated by echocardiography and cardiac catheterization. Hearts were later excised for histological and immunostaining analysis. Left ventricular function was significantly better in BMSC-transplanted animals than in controls. Cellular staining of BrdU and N-cadherin, indicating the presence of bone-marrow-derived cells, was found in BMSC-transplanted animals but not in control and sham-operated animals. CONCLUSIONS: BMSC transplantation can improve left ventricular function in DCM. Direct injection into the ventricular wall is an effective approach for stem cell delivery. Crown Copyright 2009. Published by Elsevier Inc. All rights reserved.
Authors: Maira S Oliveira; Marcos B Melo; Juliana L Carvalho; Isabela M Melo; Mario Sl Lavor; Dawidson A Gomes; Alfredo M de Goes; Marilia M Melo Journal: J Cancer Sci Ther Date: 2013
Authors: Miguel Romero; José Suárez-de-Lezo; Concha Herrera; Manuel Pan; José López-Aguilera; José Suárez-de-Lezo; Flor Baeza-Garzón; Francisco Javier Hidalgo-Lesmes; Olga Fernández-López; Juliana Martínez-Atienza; Eva Cebrián; Vanesa Martín-Palanco; Rosario Jiménez-Moreno; Rosario Gutiérrez-Fernández; Sonia Nogueras; Maria Dolores Carmona; Soledad Ojeda; Natividad Cuende; Rosario Mata Journal: BMC Cardiovasc Disord Date: 2019-08-22 Impact factor: 2.298