| Literature DB >> 20005203 |
Hassan Al-Ali1, Megan E Rieger, Kenneth L Seldeen, Thomas K Harris, Amjad Farooq, Karoline J Briegel.
Abstract
Limb-bud and heart (LBH) is a key transcriptional regulator in vertebrates with pivotal roles in embryonic development and human disease. Herein, using a diverse array of biophysical techniques, we report the first structural characterization of LBH pertinent to its biological function. Our data reveal that LBH is structurally disordered with no discernable secondary or tertiary structure and exudes rod-like properties in solution. Consistent with these observations, we also demonstrate that LBH is conformationally flexible and thus may be capable of adapting distinct conformations under specific physiological contexts. We propose that LBH is a member of the intrinsically disordered protein (IDP) family, and that conformational plasticity may play a significant role in modulating LBH-dependent transcriptional processes. Copyright 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 20005203 PMCID： PMC2827303 DOI： 10.1016/j.bbrc.2009.12.032
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575