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Literature DB >> 20004690

A simple and rapid method of bacterial transformation.

Gottfried Wilharm¹, Daniela Lepka, Franziska Faber, Julia Hofmann, Tobias Kerrinnes, Evelyn Skiebe.

Abstract

Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar. Copyright 2009 Elsevier B.V. All rights reserved.

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Year: 2010 PMID： 20004690 DOI： 10.1016/j.mimet.2009.12.002

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

8 in total

Review 1. Manipulating Bacterial Communities by in situ Microbiome Engineering.

Authors: Ravi U Sheth; Vitor Cabral; Sway P Chen; Harris H Wang
Journal: Trends Genet Date: 2016-02-22 Impact factor: 11.639

2. The animal food supplement sepiolite promotes a direct horizontal transfer of antibiotic resistance plasmids between bacterial species.

Authors: Jerónimo Rodríguez-Beltrán; Alexandro Rodríguez-Rojas; Elva Yubero; Jesús Blázquez
Journal: Antimicrob Agents Chemother Date: 2013-03-25 Impact factor: 5.191

8. Subminimal inhibitory concentrations of ampicillin and mechanical stimuli cooperatively promote cell-to-cell plasmid transformation in Escherichia coli.

Authors: Sayuri Kasagaki; Mayuko Hashimoto; Sumio Maeda
Journal: Curr Res Microb Sci Date: 2022-03-26

8 in total

A simple and rapid method of bacterial transformation.

Review 1. Manipulating Bacterial Communities by in situ Microbiome Engineering.

2. The animal food supplement sepiolite promotes a direct horizontal transfer of antibiotic resistance plasmids between bacterial species.

3. Optimization of bacterial plasmid transformation using nanomaterials based on the Yoshida effect.

4. A simple and non-invasive method for nuclear transformation of intact-walled Chlamydomonas reinhardtii.

5. Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement.

6. Clay-induced DNA breaks as a path for genetic diversity, antibiotic resistance, and asbestos carcinogenesis.

Review 7. Approaches to genetic tool development for rapid domestication of non-model microorganisms.

8. Subminimal inhibitory concentrations of ampicillin and mechanical stimuli cooperatively promote cell-to-cell plasmid transformation in Escherichia coli.