AIMS: The aim of this work was to investigate the possible effect of human cathelicidin antimicrobial peptide LL37 on biofilm formation of Staphylococcus epidermidis, a major causative agent of indwelling device-related infections. METHODS AND RESULTS: We performed initial attachment assay and biofilm formation solid surface assay in microtitre plates, as well as growth experiment in liquid medium using laboratory strain Staph. epidermidis ATCC35984. We found that already a low concentration of the peptide LL37 (1 mg l(-1)) significantly decreased both the attachment of bacteria to the surface and also the biofilm mass. No growth inhibition was observed even at 16 mg l(-1) concentration of LL37, indicating a direct effect of the peptide on biofilm production. CONCLUSIONS: As biofilm protects bacteria during infections in humans and allows their survival in a hostile environment, inhibition of biofilm formation by LL37 may have a key role to prevent bacterial colonization on indwelling devices. SIGNIFICANCE AND IMPACT OF THE STUDY: Our findings suggest that this host defence factor can be a potential candidate in prevention and treatment strategies of Staph. epidermidis infections in humans.
AIMS: The aim of this work was to investigate the possible effect of human cathelicidin antimicrobial peptide LL37 on biofilm formation of Staphylococcus epidermidis, a major causative agent of indwelling device-related infections. METHODS AND RESULTS: We performed initial attachment assay and biofilm formation solid surface assay in microtitre plates, as well as growth experiment in liquid medium using laboratory strain Staph. epidermidis ATCC35984. We found that already a low concentration of the peptide LL37 (1 mg l(-1)) significantly decreased both the attachment of bacteria to the surface and also the biofilm mass. No growth inhibition was observed even at 16 mg l(-1) concentration of LL37, indicating a direct effect of the peptide on biofilm production. CONCLUSIONS: As biofilm protects bacteria during infections in humans and allows their survival in a hostile environment, inhibition of biofilm formation by LL37 may have a key role to prevent bacterial colonization on indwelling devices. SIGNIFICANCE AND IMPACT OF THE STUDY: Our findings suggest that this host defence factor can be a potential candidate in prevention and treatment strategies of Staph. epidermidis infections in humans.
Authors: Nicolas Delattin; Katrijn De Brucker; David J Craik; Olivier Cheneval; Mirjam Fröhlich; Matija Veber; Lenart Girandon; Talya R Davis; Anne E Weeks; Carol A Kumamoto; Paul Cos; Tom Coenye; Barbara De Coninck; Bruno P A Cammue; Karin Thevissen Journal: Antimicrob Agents Chemother Date: 2014-02-24 Impact factor: 5.191
Authors: Katrijn De Brucker; Nicolas Delattin; Stijn Robijns; Hans Steenackers; Natalie Verstraeten; Bart Landuyt; Walter Luyten; Liliane Schoofs; Barbara Dovgan; Mirjam Fröhlich; Jan Michiels; Jos Vanderleyden; Bruno P A Cammue; Karin Thevissen Journal: Antimicrob Agents Chemother Date: 2014-06-30 Impact factor: 5.191
Authors: Berthony Deslouches; Mary L Hasek; Jodi K Craigo; Jonathan D Steckbeck; Ronald C Montelaro Journal: J Med Microbiol Date: 2016-04-05 Impact factor: 2.472
Authors: Lauren P Lashua; Jeffrey A Melvin; Berthony Deslouches; Joseph M Pilewski; Ronald C Montelaro; Jennifer M Bomberger Journal: J Antimicrob Chemother Date: 2016-05-26 Impact factor: 5.790