| Literature DB >> 19995580 |
Richard de Boer1, Remco Peters, Sonja Gierveld, Tim Schuurman, Mirjam Kooistra-Smid, Paul Savelkoul.
Abstract
Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics. Copyright 2009 Elsevier B.V. All rights reserved.Entities:
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Year: 2009 PMID： 19995580 DOI： 10.1016/j.mimet.2009.11.009
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363