| Literature DB >> 19966415 |
Justyna A Wojdyla1, Ioannis Manolaridis, Eric J Snijder, Alexander E Gorbalenya, Bruno Coutard, Yvonne Piotrowski, Rolf Hilgenfeld, Paul A Tucker.
Abstract
The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 A resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.Entities:
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Year: 2009 PMID： 19966415 PMCID： PMC7161637 DOI： 10.1107/S0907444909040074
Source DB: PubMed Journal: Acta Crystallogr D Biol Crystallogr ISSN： 0907-4449