AIMS: We investigated the contribution of the cholinergic nervous system to dexamethasone-induced insulin resistance and hyperinsulinemia in rats. METHODS: Seventy-day-old Wistar male rats were distributed in groups: control (CTL), vagotomized (VAG), and sham operated (SHAM). On the 90th day of life, half of the rats were treated daily with 1mg/kg of dexamethasone for 5 days (CTL DEX, VAG DEX, and SHAM DEX). RESULTS: In the presence of 8.3mM glucose plus 100microM carbachol (Cch), isolated islets from CTL DEX secreted significantly more insulin than CTL. Cch-enhancement of secretion was further increased in islets from VAG CTL and VAG DEX than SHAM CTL and SHAM DEX, respectively. In CTL DEX islets, M3R and PLCbeta1 and phosphorylated PKCalpha, but not PKCalpha, protein content was significantly higher compared with each respective control. In islets from VAG DEX, the expression of M3R protein increased significantly compared to VAG CTL and SHAM DEX. Vagotomy per se did not affect insulin resistance, but attenuated fasted and fed insulinemia in VAG DEX, compared with SHAM DEX rats. CONCLUSION: These data indicate an important participation of the cholinergic nervous system through muscaric receptors in dexamethasone-induced hyperinsulinemia in rats. 2009 Elsevier Ireland Ltd. All rights reserved.
AIMS: We investigated the contribution of the cholinergic nervous system to dexamethasone-induced insulin resistance and hyperinsulinemia in rats. METHODS: Seventy-day-old Wistar male rats were distributed in groups: control (CTL), vagotomized (VAG), and sham operated (SHAM). On the 90th day of life, half of the rats were treated daily with 1mg/kg of dexamethasone for 5 days (CTL DEX, VAG DEX, and SHAM DEX). RESULTS: In the presence of 8.3mM glucose plus 100microM carbachol (Cch), isolated islets from CTL DEX secreted significantly more insulin than CTL. Cch-enhancement of secretion was further increased in islets from VAG CTL and VAG DEX than SHAM CTL and SHAM DEX, respectively. In CTL DEX islets, M3R and PLCbeta1 and phosphorylated PKCalpha, but not PKCalpha, protein content was significantly higher compared with each respective control. In islets from VAG DEX, the expression of M3R protein increased significantly compared to VAG CTL and SHAM DEX. Vagotomy per se did not affect insulin resistance, but attenuated fasted and fed insulinemia in VAG DEX, compared with SHAM DEXrats. CONCLUSION: These data indicate an important participation of the cholinergic nervous system through muscaric receptors in dexamethasone-induced hyperinsulinemia in rats. 2009 Elsevier Ireland Ltd. All rights reserved.
Authors: Bruna B Bighetti; Gerson F d Assis; Danilo C Vieira; Natalia M Violato; Tania M Cestari; Rumio Taga; José R Bosqueiro; Alex Rafacho Journal: Int J Exp Pathol Date: 2014-09-03 Impact factor: 1.925
Authors: Claudinéia C S Franco; Kelly V Prates; Carina Previate; Ana M P Moraes; Camila C I Matiusso; Rosiane A Miranda; Júlio C de Oliveira; Laize P Tófolo; Isabela P Martins; Luiz F Barella; Tatiane A Ribeiro; Ananda Malta; Audrei Pavanello; Flávio A Francisco; Rodrigo M Gomes; Vander S Alves; Veridiana M Moreira; Késia P Rigo; Douglas L Almeida; Juliane R de Sant Anna; Marialba A A C Prado; Paulo C F Mathias Journal: Endocrine Date: 2017-02-23 Impact factor: 3.633
Authors: Paola M Sulis; Katia Motta; Amanda M Barbosa; Matheus H Besen; Julia S da Silva; Everson A Nunes; Alex Rafacho Journal: Biomed Res Int Date: 2017-08-08 Impact factor: 3.411
Authors: Felipe Azevedo Gomes; Rafael Appel Flores; Maciel Alencar Bruxel; Flávia Natividade da Silva; Eduardo Luiz Gasnhar Moreira; Daniel Breseghello Zoccal; Rui Daniel Prediger; Alex Rafacho Journal: Front Neurosci Date: 2019-01-09 Impact factor: 4.677