| Literature DB >> 19961882 |
M Sudhadham1, G S de Hoog, S B J Menken, A H G Gerrits van den Ende, P Sihanonth.
Abstract
A simple method for fungal genotype screening was developed for the black yeast Exophiala dermatitidis based on RFLP of ribosomal ITS regions currently used as potential virulence markers. In a study set of 502 strains of the species, two main genotypes were recognized. Only 0.97% of lanes were difficult to interpret as they did not clearly present one of the expected genotypes. Twenty strains were deviating and proved to be E. spinifera after sequencing. Eight common, related species (based on SSU data) with clinical significance yielded different patterns with TaqI digestion, and thus the method is also usable for routine diagnostics. Copyright 2009. Published by Elsevier B.V.Entities:
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Year: 2009 PMID： 19961882 DOI： 10.1016/j.mimet.2009.11.007
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363