PURPOSE: To determine the in vivo efficacy of cyclosporine A-loaded solid lipid nanoparticles (SLNs) in rabbit eyes. METHODS: SLNs were prepared and administered to the cul-de-sac of rabbits, and the drug amount in aqueous humor was detected by high performance liquid chromatography (HPLC). The irritation was evaluated by modified Draize testing. RESULTS: The particle size of SLNs was detected as 225.9 +/- 5.5 nm with a negative surface charge. Aqueous humor drug levels reached 50.53 ng/mL, and there was no serious irritation in rabbit eyes. CONCLUSIONS: Topical ophthalmic efficacy of cyclosporine A was enhanced via administration of SLNs.
PURPOSE: To determine the in vivo efficacy of cyclosporine A-loaded solid lipid nanoparticles (SLNs) in rabbit eyes. METHODS: SLNs were prepared and administered to the cul-de-sac of rabbits, and the drug amount in aqueous humor was detected by high performance liquid chromatography (HPLC). The irritation was evaluated by modified Draize testing. RESULTS: The particle size of SLNs was detected as 225.9 +/- 5.5 nm with a negative surface charge. Aqueous humor drug levels reached 50.53 ng/mL, and there was no serious irritation in rabbit eyes. CONCLUSIONS: Topical ophthalmic efficacy of cyclosporine A was enhanced via administration of SLNs.
Authors: Hamideh Parhiz; Makan Khoshnejad; Jacob W Myerson; Elizabeth Hood; Priyal N Patel; Jacob S Brenner; Vladimir R Muzykantov Journal: Adv Drug Deliv Rev Date: 2018-07-03 Impact factor: 15.470
Authors: I Bravo-Osuna; V Andrés-Guerrero; P Pastoriza Abal; I T Molina-Martínez; R Herrero-Vanrell Journal: Drug Deliv Transl Res Date: 2016-12 Impact factor: 4.617
Authors: Carmelo Puglia; Paolo Blasi; Carmine Ostacolo; Eduardo Sommella; Claudio Bucolo; Chiara B M Platania; Giovanni L Romano; Federica Geraci; Filippo Drago; Debora Santonocito; Barbara Albertini; Pietro Campiglia; Giovanni Puglisi; Rosario Pignatello Journal: Front Pharmacol Date: 2018-03-28 Impact factor: 5.810