| Literature DB >> 1995702 |
E Juronen1, J Parik, P Toomik.
Abstract
Two fast methods for the purification of mouse monoclonal antibodies from ascites, fluids using Blue-DEAE and 'thiophilic' adsorbent (T gel) in the FPLC system are described. Blue-DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory adsorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.Entities:
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Year: 1991 PMID： 1995702 DOI： 10.1016/0022-1759(91)90255-e
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303