BACKGROUND: Aberrant DNA methylation has been recognized in human breast carcinogenesis as a common molecular alteration associated with the loss of expression of a number of key regulatory genes. The present study was undertaken to determine whether methylation and expression of p16 and FHIT genes would correlate with the estrogen receptor (ER) and progesterone receptor (PR) status. METHODS: Methylation-specific polymerase chain reaction, messenger RNA (mRNA) expression analysis, immunohistochemistry, and Western blot analysis were performed to study the methylation of p16 and FHIT genes in 351 pairs of malignant/normal breast tissues. We examined the expression of ER and PR in those specimens by immunohistochemistry. Mutations of p16 and FHIT genes in tumors were detected by direct sequencing. RESULTS: The frequency of hypermethylation was 31.9% and 36.8% in p16 and FHIT genes, respectively, and showed significant harmony in concordant hypermethylation (P < .0001). In postmenopausal patients, methylation frequency in both genes is significantly higher in poorly and moderately differentiated tumors. Loss of protein expression of p16 and FHIT in 77 and 74 tumors, respectively, is associated with their methylation status in premenopausal women. CONCLUSION: We did not find any significant differences in tumor-related gene methylation patterns relevant to both ER and PR status of breast tumors.
BACKGROUND: Aberrant DNA methylation has been recognized in humanbreast carcinogenesis as a common molecular alteration associated with the loss of expression of a number of key regulatory genes. The present study was undertaken to determine whether methylation and expression of p16 and FHIT genes would correlate with the estrogen receptor (ER) and progesterone receptor (PR) status. METHODS: Methylation-specific polymerase chain reaction, messenger RNA (mRNA) expression analysis, immunohistochemistry, and Western blot analysis were performed to study the methylation of p16 and FHIT genes in 351 pairs of malignant/normal breast tissues. We examined the expression of ER and PR in those specimens by immunohistochemistry. Mutations of p16 and FHIT genes in tumors were detected by direct sequencing. RESULTS: The frequency of hypermethylation was 31.9% and 36.8% in p16 and FHIT genes, respectively, and showed significant harmony in concordant hypermethylation (P < .0001). In postmenopausal patients, methylation frequency in both genes is significantly higher in poorly and moderately differentiated tumors. Loss of protein expression of p16 and FHIT in 77 and 74 tumors, respectively, is associated with their methylation status in premenopausal women. CONCLUSION: We did not find any significant differences in tumor-related gene methylation patterns relevant to both ER and PR status of breast tumors.
Authors: Meng Hua Tao; Peter G Shields; Jing Nie; Amy Millen; Christine B Ambrosone; Stephen B Edge; Shiva S Krishnan; Catalin Marian; Bin Xie; Janet Winston; Dominica Vito; Maurizio Trevisan; Jo L Freudenheim Journal: Breast Cancer Res Treat Date: 2008-05-08 Impact factor: 4.872
Authors: Catherine L Callahan; Youjin Wang; Catalin Marian; Daniel Y Weng; Kevin H Eng; Meng-Hua Tao; Christine B Ambrosone; Jing Nie; Maurizio Trevisan; Dominic Smiraglia; Stephen B Edge; Peter G Shields; Jo L Freudenheim Journal: Epigenetics Date: 2016-05-31 Impact factor: 4.528
Authors: Kapil Bhatia; Pratibha Misra; Yan Naing Soe; M K Sibin; H S Batra; Divya Shelly; Sangeetha Sampath; Rakhi Negi; Bhasker Mukherjee; Rajat Jagani Journal: Med J Armed Forces India Date: 2021-09-30