Yi Miao1, Bin Cai, Ling Liu, Yixia Yang, Xiaoping Wan. 1. Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, People's Republic of China.
Abstract
OBJECTIVE: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray. METHODS: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC. RESULTS: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray. CONCLUSIONS: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.
OBJECTIVE: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray. METHODS: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC. RESULTS: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray. CONCLUSIONS: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.
Authors: Danhua Zhang; Vladislav S Golubkov; Wenlong Han; Ricardo G Correa; Ying Zhou; Sunyoung Lee; Alex Y Strongin; P Duc Si Dong Journal: Dev Biol Date: 2014-08-29 Impact factor: 3.582
Authors: Angela M Hancock; David B Witonsky; Gorka Alkorta-Aranburu; Cynthia M Beall; Amha Gebremedhin; Rem Sukernik; Gerd Utermann; Jonathan K Pritchard; Graham Coop; Anna Di Rienzo Journal: PLoS Genet Date: 2011-04-21 Impact factor: 5.917