Ayinuer Adijiang1, Toshimitsu Niwa. 1. Clinical Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan.
Abstract
BACKGROUND/AIM: Klotho, an anti-aging gene, is primarily expressed in the kidney, and its renal expression is decreased in chronic renal failure (CRF). We determined if administration of an oral sorbent, AST-120, increases the expression of Klotho, and inhibits cell senescence in the kidney of CRF rats. METHODS: CRF rats were produced by 4/5-nephrectomy. AST-120 was administered to the CRF rats at a dose of 4 g/kg with powder chow for 16 weeks, whereas powder chow alone was administered to control CRF rats. The expression of Klotho and cell senescence (senescence-associated beta-galactosidase: SA-beta-gal) was detected by immunohistochemistry. RESULTS: The expression of Klotho was significantly decreased in the kidney of CRF rats as compared with normal rats. AST-120-treated CRF rats showed significantly increased renal expression of Klotho as compared with CRF rats. The expression of SA-beta-gal was significantly increased in the kidney of CRF rats as compared with normal rats. AST-120-treated CRF rats showed significantly decreased expression of SA-beta-gal in the kidney as compared with CRF rats. AST-120 significantly decreased serum and urine levels of indoxyl sulfate. CONCLUSIONS: AST-120 increased Klotho expression, and inhibited cell senescence in the kidney of CRF rats, probably by alleviating indoxyl sulfate overload on the kidney. Copyright (c) 2009 S. Karger AG, Basel.
BACKGROUND/AIM: Klotho, an anti-aging gene, is primarily expressed in the kidney, and its renal expression is decreased in chronic renal failure (CRF). We determined if administration of an oral sorbent, AST-120, increases the expression of Klotho, and inhibits cell senescence in the kidney of CRF rats. METHODS: CRF rats were produced by 4/5-nephrectomy. AST-120 was administered to the CRF rats at a dose of 4 g/kg with powder chow for 16 weeks, whereas powder chow alone was administered to control CRF rats. The expression of Klotho and cell senescence (senescence-associated beta-galactosidase: SA-beta-gal) was detected by immunohistochemistry. RESULTS: The expression of Klotho was significantly decreased in the kidney of CRF rats as compared with normal rats. AST-120-treated CRF rats showed significantly increased renal expression of Klotho as compared with CRF rats. The expression of SA-beta-gal was significantly increased in the kidney of CRF rats as compared with normal rats. AST-120-treated CRF rats showed significantly decreased expression of SA-beta-gal in the kidney as compared with CRF rats. AST-120 significantly decreased serum and urine levels of indoxyl sulfate. CONCLUSIONS: AST-120 increased Klotho expression, and inhibited cell senescence in the kidney of CRF rats, probably by alleviating indoxyl sulfate overload on the kidney. Copyright (c) 2009 S. Karger AG, Basel.
Authors: Gwendalyn D King; CiDi Chen; Mickey M Huang; Ella Zeldich; Patricia L Brazee; Eli R Schuman; Maxime Robin; Gregory D Cuny; Marcie A Glicksman; Carmela R Abraham Journal: Biochem J Date: 2012-01-01 Impact factor: 3.857
Authors: Ran-Hui Cha; Shin Wook Kang; Cheol Whee Park; Dae Ryong Cha; Ki Young Na; Sung Gyun Kim; Sun Ae Yoon; Sang Youb Han; Jae Hyun Chang; Sue K Park; Chun Soo Lim; Yon Su Kim Journal: Clin J Am Soc Nephrol Date: 2016-02-09 Impact factor: 8.237