Literature DB >> 19948855

Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.

Gerard A Cangelosi1, Kris M Weigel, Clarita Lefthand-Begay, John S Meschke.   

Abstract

Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.

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Year:  2009        PMID： 19948855      PMCID： PMC2812999          DOI： 10.1128/AEM.01810-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

1.  Depletion of pre-16S rRNA in starved Escherichia coli cells.

Authors:  G A Cangelosi; W H Brabant
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

2.  The two-component regulatory system mtrAB is required for morphotypic multidrug resistance in Mycobacterium avium.

Authors:  Gerard A Cangelosi; Julie S Do; Robert Freeman; John G Bennett; Makeda Semret; Marcel A Behr
Journal:  Antimicrob Agents Chemother       Date:  2006-02       Impact factor: 5.191

3.  Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms.

Authors:  L K Poulsen; G Ballard; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792

4.  Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin.

Authors:  D R Sherman; M Voskuil; D Schnappinger; R Liao; M I Harrell; G K Schoolnik
Journal:  Proc Natl Acad Sci U S A       Date:  2001-06-19       Impact factor: 11.205

5.  Inhibition of Escherichia coli precursor-16S rRNA processing by mouse intestinal contents.

Authors:  T R Licht; T Tolker-Nielsen; K Holmstrøm; K A Krogfelt; S Molin
Journal:  Environ Microbiol       Date:  1999-02       Impact factor: 5.491

6.  Potential virulence of viable but nonculturable Shigella dysenteriae type 1.

Authors:  I Rahman; M Shahamat; M A Chowdhury; R R Colwell
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

7.  Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio.

Authors: 
Journal:  Microb Ecol       Date:  1999-11       Impact factor: 4.552

8.  The Mycobacterium tuberculosis complex transcriptome of attenuation.

Authors:  Serge Mostowy; Cynthia Cleto; David R Sherman; Marcel A Behr
Journal:  Tuberculosis (Edinb)       Date:  2004       Impact factor: 3.131

9.  Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period.

Authors:  Kathleen L Horan; Robert Freeman; Kris Weigel; Makeda Semret; Stacy Pfaller; Terry C Covert; Dick van Soolingen; Sylvia C Leão; Marcel A Behr; Gerard A Cangelosi
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

10.  Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples.

Authors:  Phillip B Gedalanga; Betty H Olson
Journal:  Appl Microbiol Biotechnol       Date:  2009-01-20       Impact factor: 4.813
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  15 in total

Review 1.  Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology.

Authors:  Lisa-Maria Mauerhofer; Patricia Pappenreiter; Christian Paulik; Arne H Seifert; Sébastien Bernacchi; Simon K-M R Rittmann
Journal:  Folia Microbiol (Praha)       Date:  2018-11-16       Impact factor: 2.099

Review 2.  Dead or alive: molecular assessment of microbial viability.

Authors:  Gerard A Cangelosi; John S Meschke
Journal:  Appl Environ Microbiol       Date:  2014-07-18       Impact factor: 4.792

3.  Differences in Temperature and Water Chemistry Shape Distinct Diversity Patterns in Thermophilic Microbial Communities.

Authors:  Cecilia M Chiriac; Edina Szekeres; Knut Rudi; Andreea Baricz; Adriana Hegedus; Nicolae Dragoş; Cristian Coman
Journal:  Appl Environ Microbiol       Date:  2017-10-17       Impact factor: 4.792

4.  Molecular Viability Testing of UV-Inactivated Bacteria.

Authors:  Kris M Weigel; Felicia K Nguyen; Moira R Kearney; John S Meschke; Gerard A Cangelosi
Journal:  Appl Environ Microbiol       Date:  2017-05-01       Impact factor: 4.792

5.  Mechanical disruption of lysis-resistant bacterial cells by use of a miniature, low-power, disposable device.

Authors:  Peter E Vandeventer; Kris M Weigel; Jose Salazar; Barbara Erwin; Bruce Irvine; Robert Doebler; Ali Nadim; Gerard A Cangelosi; Angelika Niemz
Journal:  J Clin Microbiol       Date:  2011-05-04       Impact factor: 5.948

6.  Evaluation of a pan-Leishmania SL RNA qPCR assay for parasite detection in laboratory-reared and field-collected sand flies and reservoir hosts.

Authors:  Myrthe Pareyn; Rik Hendrickx; Nigatu Girma; Sarah Hendrickx; Lieselotte Van Bockstal; Natalie Van Houtte; Simon Shibru; Louis Maes; Herwig Leirs; Guy Caljon
Journal:  Parasit Vectors       Date:  2020-06-01       Impact factor: 3.876

7.  Mycobacterium tuberculosis precursor rRNA as a measure of treatment-shortening activity of drugs and regimens.

Authors:  Nicholas D Walter; Sarah E M Born; Gregory T Robertson; Matthew Reichlen; Christian Dide-Agossou; Victoria A Ektnitphong; Karen Rossmassler; Michelle E Ramey; Allison A Bauman; Victor Ozols; Shelby C Bearrows; Gary Schoolnik; Gregory Dolganov; Benjamin Garcia; Emmanuel Musisi; William Worodria; Laurence Huang; J Lucian Davis; Nhung V Nguyen; Hung V Nguyen; Anh T V Nguyen; Ha Phan; Carol Wilusz; Brendan K Podell; N' Dira Sanoussi; Bouke C de Jong; Corinne S Merle; Dissou Affolabi; Helen McIlleron; Maria Garcia-Cremades; Ekaterina Maidji; Franceen Eshun-Wilson; Brandon Aguilar-Rodriguez; Dhuvarakesh Karthikeyan; Khisimuzi Mdluli; Cathy Bansbach; Anne J Lenaerts; Radojka M Savic; Payam Nahid; Joshua J Vásquez; Martin I Voskuil
Journal:  Nat Commun       Date:  2021-05-18       Impact factor: 14.919

8.  Steady-State Pre-rRNA Analysis to Investigate the Functional Microbiome.

Authors:  Kris M Weigel; Alaina M Olson; Gerard A Cangelosi
Journal:  Curr Protoc       Date:  2021-07

9.  Molecular viability testing of bacterial pathogens from a complex human sample matrix.

Authors:  Kris M Weigel; Kelly L Jones; Julie S Do; Jody Melton Witt; Jae-Hyun Chung; Christian Valcke; Gerard A Cangelosi
Journal:  PLoS One       Date:  2013-01-24       Impact factor: 3.240

10.  Biosynthetic enhancement of the detection of bacteria by the polymerase chain reaction.

Authors:  Julie S Do; Kris M Weigel; John S Meschke; Gerard A Cangelosi
Journal:  PLoS One       Date:  2014-01-17       Impact factor: 3.240
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