OBJECTIVE: To determine the value of amniotic fluid interleukin-6 (AF IL-6) and tumor necrosis factor-alpha (AF TNF-alpha) in the diagnosis of microbial invasion of the amniotic cavity and in the prediction of preterm delivery (PTD). STUDY DESIGN: Following amniocentesis, a sample of amniotic fluid was sent for aerobic and anaerobic bacterial cultures along with Ureaplasma urealyticum culture and it was also assessed for IL-6 and TNF-alpha. RESULTS: Forty-eight women who delivered preterm (<37 weeks) were matched with 96 controls. The AF IL-6 and TNF-alpha concentrations of women with spontaneous PTD were significantly higher than those who delivered at term (IL-6: 176.3 pg/ml [130.6-208.6] vs. 52.3 pg/ml [37.2-92.3]; TNF-alpha: 8.8 pg/ml [7.2-10.7] vs. 5.5 pg/ml [5.0-6.3]). AF IL-6 and TNF-alpha concentrations of >99.3 pg/ml and of >6.6 pg/ml respectively, had a sensitivity of 89.6% and 81.3% and a specificity of 80.3% and 79.2% for the prediction of spontaneous PTD. Moreover, AF IL-6 and TNF-alpha concentrations of >99.3 pg/ml and of 6.3 pg/ml respectively, had a sensitivity of 91.9% and 78.4% and a specificity of 73.8% and 70.1% for the prediction of a positive AF culture. CONCLUSIONS: Elevated mid-trimester concentrations of AF IL-6, or/and of TNF-alpha can identify women at risk for intra-amniotic infection and for spontaneous PTD. 2009 Elsevier Ireland Ltd. All rights reserved.
OBJECTIVE: To determine the value of amniotic fluid interleukin-6 (AF IL-6) and tumor necrosis factor-alpha (AFTNF-alpha) in the diagnosis of microbial invasion of the amniotic cavity and in the prediction of preterm delivery (PTD). STUDY DESIGN: Following amniocentesis, a sample of amniotic fluid was sent for aerobic and anaerobic bacterial cultures along with Ureaplasma urealyticum culture and it was also assessed for IL-6 and TNF-alpha. RESULTS: Forty-eight women who delivered preterm (<37 weeks) were matched with 96 controls. The AFIL-6 and TNF-alpha concentrations of women with spontaneous PTD were significantly higher than those who delivered at term (IL-6: 176.3 pg/ml [130.6-208.6] vs. 52.3 pg/ml [37.2-92.3]; TNF-alpha: 8.8 pg/ml [7.2-10.7] vs. 5.5 pg/ml [5.0-6.3]). AFIL-6 and TNF-alpha concentrations of >99.3 pg/ml and of >6.6 pg/ml respectively, had a sensitivity of 89.6% and 81.3% and a specificity of 80.3% and 79.2% for the prediction of spontaneous PTD. Moreover, AFIL-6 and TNF-alpha concentrations of >99.3 pg/ml and of 6.3 pg/ml respectively, had a sensitivity of 91.9% and 78.4% and a specificity of 73.8% and 70.1% for the prediction of a positive AF culture. CONCLUSIONS: Elevated mid-trimester concentrations of AFIL-6, or/and of TNF-alpha can identify women at risk for intra-amniotic infection and for spontaneous PTD. 2009 Elsevier Ireland Ltd. All rights reserved.
Authors: Roberto Romero; Zeynep Alpay Savasan; Tinnakorn Chaiworapongsa; Stanley M Berry; Juan Pedro Kusanovic; Sonia S Hassan; Bo Hyun Yoon; Samuel Edwin; Moshe Mazor Journal: J Perinat Med Date: 2011-09-30 Impact factor: 1.901
Authors: Percy Pacora; Roberto Romero; Offer Erez; Eli Maymon; Bogdan Panaitescu; Juan Pedro Kusanovic; Adi L Tarca; Chaur-Dong Hsu; Sonia S Hassan Journal: J Matern Fetal Neonatal Med Date: 2017-12-27
Authors: Chen Xu; Weina Liu; Xingji You; Kelycia Leimert; Krystyn Popowycz; Xin Fang; Stephen L Wood; Donna M Slater; Qianqian Sun; Hang Gu; David M Olson; Xin Ni Journal: Mol Hum Reprod Date: 2015-04-16 Impact factor: 4.025