| Literature DB >> 19944062 |
Duska Separovic1, Ameeta Kelekar, Arun K Nayak, Adi L Tarca, Kentaro Hanada, Jason S Pierce, Jacek Bielawski.
Abstract
The purpose of this study was to determine the sphingolipid (SL) profile in cells defective in autophagy protein ATG-7 and overall cell death after photodynamic therapy (PDT) with the photosensitizer Pc 4. MCF-7 human breast cancer cells with downregulated ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to increased cell killing. PDT evoked an early (2h) greater global increase in ceramides in ATG-7 defective cells compared to Scr cells. The total increases in dihydroceramide (DHceramide) were significant at 2 and 24h in both cell types post-PDT. The levels of sphingosine-1-phosphate (S1P) and sphingosine were decreased below resting levels at both time points irrespective of the cell type. The data imply that ceramide might be a marker of ATG-7 deficiency in cells sensitized to PDT. Copyright (c) 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 19944062 PMCID： PMC2877393 DOI： 10.1016/j.abb.2009.11.023
Source DB: PubMed Journal: Arch Biochem Biophys ISSN： 0003-9861 Impact factor: 4.013