PURPOSE: Tissue-engineered cartilage may offer a solution for the treatment of serious airway disease. This study developed a novel procedure to fabricate a scaffold-free cylindrical cartilage under in vitro conditions, while also evaluating the effect of a dynamic culture on the engineered construct. METHODS: Auricular chondrocytes were harvested from New Zealand white rabbits and cultivated under high-density conditions to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated for 6 weeks in dynamic or static conditions. The engineered cylindrical cartilages were evaluated macroscopically and histologically. The expression of collagen, glycosaminoglycan content and mechanical properties were determined. RESULTS: The cylindrical cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Histologically, the construct contained a Safranin-O positive cartilaginous matrix accompanied by the expression of type II collagen. The glycosaminoglycan content increased and reached 72% of the native tracheal cartilage after 6 weeks of cultivation. CONCLUSION: A novel procedure was developed for fabricating engineered cartilage, which maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions using sheet-based tissue engineering techniques. This procedure may allow for the development of a tailor-made autograft and a functionally engineered trachea.
PURPOSE: Tissue-engineered cartilage may offer a solution for the treatment of serious airway disease. This study developed a novel procedure to fabricate a scaffold-free cylindrical cartilage under in vitro conditions, while also evaluating the effect of a dynamic culture on the engineered construct. METHODS: Auricular chondrocytes were harvested from New Zealand white rabbits and cultivated under high-density conditions to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated for 6 weeks in dynamic or static conditions. The engineered cylindrical cartilages were evaluated macroscopically and histologically. The expression of collagen, glycosaminoglycan content and mechanical properties were determined. RESULTS: The cylindrical cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Histologically, the construct contained a Safranin-O positive cartilaginous matrix accompanied by the expression of type II collagen. The glycosaminoglycan content increased and reached 72% of the native tracheal cartilage after 6 weeks of cultivation. CONCLUSION: A novel procedure was developed for fabricating engineered cartilage, which maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions using sheet-based tissue engineering techniques. This procedure may allow for the development of a tailor-made autograft and a functionally engineered trachea.
Authors: G Schulze-Tanzil; P de Souza; H Villegas Castrejon; T John; H-J Merker; A Scheid; M Shakibaei Journal: Cell Tissue Res Date: 2002-05-18 Impact factor: 5.249
Authors: Nicolas L'Heureux; Nathalie Dusserre; Gerhardt Konig; Braden Victor; Paul Keire; Thomas N Wight; Nicolas A F Chronos; Andrew E Kyles; Clare R Gregory; Grant Hoyt; Robert C Robbins; Todd N McAllister Journal: Nat Med Date: 2006-02-19 Impact factor: 53.440
Authors: Koji Kojima; Lawrence J Bonassar; Amit K Roy; Charles A Vacanti; Joaquin Cortiella Journal: J Thorac Cardiovasc Surg Date: 2002-06 Impact factor: 5.209
Authors: James E Dennis; Kristina G Bernardi; Thomas J Kean; Nelson E Liou; Tanya K Meyer Journal: J Tissue Eng Regen Med Date: 2017-11-10 Impact factor: 3.963