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Literature DB >> 19942433

A single step purification for autolytic zinc proteinases.

Erin M Wilfong¹, Ursala Locklear, Eric J Toone.

Abstract

We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases. Copyright 2009 Elsevier Ltd. All rights reserved.

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Year: 2009 PMID： 19942433 PMCID： PMC3688063 DOI： 10.1016/j.bmcl.2009.10.114

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

27 in total

1. Design and therapeutic application of matrix metalloproteinase inhibitors.

Authors: M Whittaker; C D Floyd; P Brown; A J Gearing
Journal: Chem Rev Date: 1999-09-08 Impact factor: 60.622

2. Human fibroblast stromelysin catalytic domain: expression, purification, and characterization of a C-terminally truncated form.

Authors: A I Marcy; L L Eiberger; R Harrison; H K Chan; N I Hutchinson; W K Hagmann; P M Cameron; D A Boulton; J D Hermes
Journal: Biochemistry Date: 1991-07-02 Impact factor: 3.162

3. Spectroscopic determination of tryptophan and tyrosine in proteins.

Authors: H Edelhoch
Journal: Biochemistry Date: 1967-07 Impact factor: 3.162

4. Cloning, expression, purification, and characterization of rat MMP-12.

Authors: J Y Fu; A Lyga; H Shi; M L Blue; B Dixon; D Chen
Journal: Protein Expr Purif Date: 2001-03 Impact factor: 1.650

5. Evaluation of the interaction of peptides with Cu(II), Ni(II), and Zn(II) by high-performance immobilized metal ion affinity chromatography.

Authors: T T Yip; Y Nakagawa; J Porath
Journal: Anal Biochem Date: 1989-11-15 Impact factor: 3.365

6. The expression, refolding, and purification of the catalytic domain of human collagenase-3 (MMP-13).

Authors: N Pathak; S I Hu; J A Koehn
Journal: Protein Expr Purif Date: 1998-11 Impact factor: 1.650

7. Disruption of the cysteine-75 and zinc ion coordination is not sufficient to activate the precursor of human matrix metalloproteinase 3 (stromelysin 1).

Authors: L C Chen; M E Noelken; H Nagase
Journal: Biochemistry Date: 1993-10-05 Impact factor: 3.162

A single step purification for autolytic zinc proteinases.

1. Design and therapeutic application of matrix metalloproteinase inhibitors.

2. Human fibroblast stromelysin catalytic domain: expression, purification, and characterization of a C-terminally truncated form.

3. Spectroscopic determination of tryptophan and tyrosine in proteins.

4. Cloning, expression, purification, and characterization of rat MMP-12.

5. Evaluation of the interaction of peptides with Cu(II), Ni(II), and Zn(II) by high-performance immobilized metal ion affinity chromatography.

6. The expression, refolding, and purification of the catalytic domain of human collagenase-3 (MMP-13).

7. Disruption of the cysteine-75 and zinc ion coordination is not sufficient to activate the precursor of human matrix metalloproteinase 3 (stromelysin 1).

8. Crystal structure of the catalytic domain of human matrix metalloproteinase 10.

9. Stromelysin-1: three-dimensional structure of the inhibited catalytic domain and of the C-truncated proenzyme.

10. Purification and characterization of the human stromelysin catalytic domain expressed in Escherichia coli.

1. A multidisciplinary approach to probing enthalpy-entropy compensation and the interfacial mobility model.

2. An enthalpic basis of additivity in biphenyl hydroxamic acid ligands for stromelysin-1.