| Literature DB >> 19940838 |
Melissa Paulite1, Zahra Fakhraai, Boris B Akhremitchev, Kerstin Mueller, Gilbert C Walker.
Abstract
This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit. The apertureless near-field microscope is a light scattering-type instrument that provides infrared spectra at circa 20 nm resolution. A complete list of components and a step-by-step protocol for use is provided. Common errors in assembly and instrument tuning are discussed. A representative data set that shows the secondary structure of an amyloid fibril is presented.Mesh:
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Year: 2009 PMID: 19940838 PMCID: PMC3304400 DOI: 10.3791/1581
Source DB: PubMed Journal: J Vis Exp ISSN: 1940-087X Impact factor: 1.355