Simultaneous spectrophotometric determination of trace amount of malachite green and crystal violet in water after cloud point extraction using partial least squares regression.

In this work, a new method has been proposed to simultaneously determine the trace amount of malachite green and crystal violet from aqueous solution by spectrophotometry after cloud point extraction (CPE) using partial least squares regression. The optimal extraction and operating conditions, such as pH, reagents concentration and effect of time and temperature, and so on, have been investigated using the non-ionic surfactant Triton X-114. The maximum absorption wavelength for malachite green and crystal violet is 624 and 579 nm, respectively; linearity is obeyed in the range of 9.9-800 and 16-1000 ng mL(-1) with detection limit of 2.9 and 4.8 ng mL(-1), and the root mean square error of prediction (RMSEP) are 0.0197 and 0.0343, respectively. The proposed method has been applied successfully to simultaneously determine the trace amount of malachite green and crystal violet in real matrix samples with the recoveries of 92.45-102.5%. (c) 2009 Elsevier B.V. All rights reserved.