OBJECTIVE: To evaluate the impact of follicle-stimulating hormone (FSH) with different glycosylation patterns on oocyte quality and clinical outcomes in an in vitro fertilization (IVF) treatment program. DESIGN: Prospective randomized, open-label, clinical study. SETTING: Assisted reproduction center. PATIENT(S): One hundred eighty-eight infertile couples undergoing assisted fertilization treatment. INTERVENTION(S): All participants underwent a standard down-regulation with a gonadotropin-releasing hormone (GnRH) analogue. The patients were randomized into three groups: 63 patients received combined sequential acidic and less-acidic FSH, 65 patients received only less-acidic FSH (recombinant FSH), and 60 received only acidic FSH (human-derived FSH). MAIN OUTCOME MEASURE(S): Number of mature metaphase II oocytes, embryo quality, clinical pregnancy rates, and implantation rates. RESULT(S): The pregnancy and implantation rates were statistically significantly lower in the less-acidic recombinant FSH alone group than in the combined sequential acidic hFSH/less-acidic recombinant FSH, and acidic hFSH alone groups (33.3%, 43.5%, 39% and 17.3%, 24.5%, 20.4%, respectively). Metaphase II oocytes and grade 1 embryos were statistically significantly higher in favor of the combined sequential acidic hFSH/less-acidic recombinant FSH group compared with the less-acidic recombinant FSH alone group. CONCLUSION(S): The glycosylation patterns of the two types of FSH implemented for ovarian stimulation have different impacts on oocyte quality and clinical outcome. A sequential combined protocol using both acidic and less-acidic FSH for ovarian stimulation improves oocyte maturity and embryo cleavage, and increases pregnancy and implantation rates.
RCT Entities:
OBJECTIVE: To evaluate the impact of follicle-stimulating hormone (FSH) with different glycosylation patterns on oocyte quality and clinical outcomes in an in vitro fertilization (IVF) treatment program. DESIGN: Prospective randomized, open-label, clinical study. SETTING: Assisted reproduction center. PATIENT(S): One hundred eighty-eight infertile couples undergoing assisted fertilization treatment. INTERVENTION(S): All participants underwent a standard down-regulation with a gonadotropin-releasing hormone (GnRH) analogue. The patients were randomized into three groups: 63 patients received combined sequential acidic and less-acidic FSH, 65 patients received only less-acidic FSH (recombinant FSH), and 60 received only acidic FSH (human-derived FSH). MAIN OUTCOME MEASURE(S): Number of mature metaphase II oocytes, embryo quality, clinical pregnancy rates, and implantation rates. RESULT(S): The pregnancy and implantation rates were statistically significantly lower in the less-acidic recombinant FSH alone group than in the combined sequential acidic hFSH/less-acidic recombinant FSH, and acidic hFSH alone groups (33.3%, 43.5%, 39% and 17.3%, 24.5%, 20.4%, respectively). Metaphase II oocytes and grade 1 embryos were statistically significantly higher in favor of the combined sequential acidic hFSH/less-acidic recombinant FSH group compared with the less-acidic recombinant FSH alone group. CONCLUSION(S): The glycosylation patterns of the two types of FSH implemented for ovarian stimulation have different impacts on oocyte quality and clinical outcome. A sequential combined protocol using both acidic and less-acidic FSH for ovarian stimulation improves oocyte maturity and embryo cleavage, and increases pregnancy and implantation rates.
Authors: Viktor Y Butnev; Vladimir Y Butnev; Jeffrey V May; Bin Shuai; Patrick Tran; William K White; Alan Brown; Aaron Smalter Hall; David J Harvey; George R Bousfield Journal: Mol Cell Endocrinol Date: 2015-02-04 Impact factor: 4.102
Authors: Chao Jiang; Xiaoying Hou; Cheng Wang; Jeffrey V May; Viktor Y Butnev; George R Bousfield; John S Davis Journal: J Clin Endocrinol Metab Date: 2015-04-27 Impact factor: 5.958