BACKGROUND: Laboratory systems to study bacterial transmission and mucosal colonization leading to infection have not been utilized. METHODS: We determined whether transmission of various strains of Pseudomonas aeruginosa among individual mice could occur and investigated the properties of such strains in establishing gastrointestinal (GI) mucosal colonization as well as in disseminating systemically after induction of neutropenia. RESULTS: P. aeruginosa isolates associated with epidemic spread among patients with cystic fibrosis (CF) readily established GI colonization at higher levels than did strains associated with acute infections in patients without CF, and they outcompeted these strains. Colonization was associated with resistance to bile salts. However, epidemic CF isolates did not disseminate after induction of neutropenia and did not induce as much mucosal pathology as did strains that were capable of disseminating. CONCLUSION: Murine models can be used to study P. aeruginosa transmission and early colonization, and the properties of these strains associated with their known clinical behaviors are mimicked in this setting.
BACKGROUND: Laboratory systems to study bacterial transmission and mucosal colonization leading to infection have not been utilized. METHODS: We determined whether transmission of various strains of Pseudomonas aeruginosa among individual mice could occur and investigated the properties of such strains in establishing gastrointestinal (GI) mucosal colonization as well as in disseminating systemically after induction of neutropenia. RESULTS:P. aeruginosa isolates associated with epidemic spread among patients with cystic fibrosis (CF) readily established GI colonization at higher levels than did strains associated with acute infections in patients without CF, and they outcompeted these strains. Colonization was associated with resistance to bile salts. However, epidemic CF isolates did not disseminate after induction of neutropenia and did not induce as much mucosal pathology as did strains that were capable of disseminating. CONCLUSION:Murine models can be used to study P. aeruginosa transmission and early colonization, and the properties of these strains associated with their known clinical behaviors are mimicked in this setting.
Authors: Y A Knirel; O V Bystrova; A S Shashkov; B Lindner; N A Kocharova; S N Senchenkova; H Moll; U Zähringer; K Hatano; G B Pier Journal: Eur J Biochem Date: 2001-09
Authors: Fadie T Coleman; Simone Mueschenborn; Gloria Meluleni; Christopher Ray; Vincent J Carey; Sara O Vargas; Carolyn L Cannon; Frederick M Ausubel; Gerald B Pier Journal: Proc Natl Acad Sci U S A Date: 2003-02-10 Impact factor: 11.205
Authors: Kalai Mathee; Giri Narasimhan; Camilo Valdes; Xiaoyun Qiu; Jody M Matewish; Michael Koehrsen; Antonis Rokas; Chandri N Yandava; Reinhard Engels; Erliang Zeng; Raquel Olavarietta; Melissa Doud; Roger S Smith; Philip Montgomery; Jared R White; Paul A Godfrey; Chinnappa Kodira; Bruce Birren; James E Galagan; Stephen Lory Journal: Proc Natl Acad Sci U S A Date: 2008-02-19 Impact factor: 11.205
Authors: David S Armstrong; Gillian M Nixon; Rosemary Carzino; Andrea Bigham; John B Carlin; Roy M Robins-Browne; Keith Grimwood Journal: Am J Respir Crit Care Med Date: 2002-10-01 Impact factor: 21.405
Authors: Irena Kukavica-Ibrulj; Alessandra Bragonzi; Moira Paroni; Craig Winstanley; François Sanschagrin; George A O'Toole; Roger C Levesque Journal: J Bacteriol Date: 2007-12-14 Impact factor: 3.490
Authors: Kelly E R Bachta; Jonathan P Allen; Bettina H Cheung; Cheng-Hsun Chiu; Alan R Hauser Journal: Nat Commun Date: 2020-01-28 Impact factor: 14.919