Xin Pu1, Yihua Yang, Shuwen Wu, Yidong Wu. 1. College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects (Ministry of Agriculture), Nanjing, PR China.
Abstract
BACKGROUND: Plutella xylostella (L.) has evolved resistance to various kinds of insecticide in the field. Reversion and selection, cross-resistance, inheritance and mechanisms of abamectin resistance were characterised in a field-derived multiresistant population of P. xylostella from China. RESULTS: Compared with a susceptible Roth strain, the field-derived TH population showed approximately 5000-fold resistance to abamectin. Rapid reversion of abamectin resistance was observed in the TH population when kept without insecticide selection. The TH-Abm strain, selected from the TH population with abamectin, developed 23 670-fold resistance to abamectin, a high level of cross-resistance to emamectin benzoate and low levels of cross-resistance to spinosad and fipronil. Genetic analyses indicated that abamectin resistance in the TH-Abm strain was autosomal, incompletely dominant and polygenic. P450 monooxygenase activities in the TH-Abm strain were significantly elevated compared with the TH strain. Piperonyl butoxide (PBO) inhibited a small part of abamectin resistance in the TH-Abm strain. CONCLUSION: Field-evolved high-level resistance to abamectin in the TH population was not stable. Selection of the TH population with abamectin resulted in an extremely high level of cross-resistance to emamectin benzoate and low levels of cross-resistance to spinosad and fipronil. Enhanced oxidative metabolism was involved in, but may not be the major mechanism of, polygenic abamectin resistance in the TH-Abm strain.
BACKGROUND:Plutella xylostella (L.) has evolved resistance to various kinds of insecticide in the field. Reversion and selection, cross-resistance, inheritance and mechanisms of abamectin resistance were characterised in a field-derived multiresistant population of P. xylostella from China. RESULTS: Compared with a susceptible Roth strain, the field-derived TH population showed approximately 5000-fold resistance to abamectin. Rapid reversion of abamectin resistance was observed in the TH population when kept without insecticide selection. The TH-Abm strain, selected from the TH population with abamectin, developed 23 670-fold resistance to abamectin, a high level of cross-resistance to emamectin benzoate and low levels of cross-resistance to spinosad and fipronil. Genetic analyses indicated that abamectin resistance in the TH-Abm strain was autosomal, incompletely dominant and polygenic. P450 monooxygenase activities in the TH-Abm strain were significantly elevated compared with the TH strain. Piperonyl butoxide (PBO) inhibited a small part of abamectin resistance in the TH-Abm strain. CONCLUSION: Field-evolved high-level resistance to abamectin in the TH population was not stable. Selection of the TH population with abamectin resulted in an extremely high level of cross-resistance to emamectin benzoate and low levels of cross-resistance to spinosad and fipronil. Enhanced oxidative metabolism was involved in, but may not be the major mechanism of, polygenic abamectin resistance in the TH-Abm strain.
Authors: Matthias B Van Hiel; Bert Breugelmans; Charles N Pagel; Adam K Williams; Aiden K Varan; Richard Burke; Vernon M Bowles; Philip Batterham Journal: PLoS One Date: 2012-11-20 Impact factor: 3.240