| Literature DB >> 1993481 |
G O Daumy1, C L Wilder, J M Merenda, A S McColl, K F Geoghegan, I G Otterness.
Abstract
A biologically active preparation of murine recombinant interleukin-1 beta (mIL-1 beta) from Escherichia coli cell lysates contained tow forms of mIL-1 beta with pI 8.7 and pI 8.1, respectively. Treatment with 0.1 M Tris, pH 8.5, at 37 degrees C for 35 h converted the pI 8.7 form to the pI 8.1 form by the selective deamidation of an asparagine residue (Asn149) in the mIL-1 beta molecule. Deamidated mIL-1 beta had 3- to 5-fold lower co-mitogenic activity and receptor affinity than the unmodified form.Entities:
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Year: 1991 PMID： 1993481 DOI： 10.1016/0014-5793(91)80093-i
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124