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Literature DB >> 19933347

Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli.

Björn Andreessen¹, Alvin Brian Lange, Horst Robenek, Alexander Steinbüchel.

Abstract

We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

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Year: 2009 PMID： 19933347 PMCID： PMC2805219 DOI： 10.1128/AEM.02097-09

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

28 in total

1. Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies.

Authors: W Yuan; Y Jia; J Tian; K D Snell; U Müh; A J Sinskey; R H Lambalot; C T Walsh; J Stubbe
Journal: Arch Biochem Biophys Date: 2001-10-01 Impact factor: 4.013

2. Application of a propionyl coenzyme A synthetase for poly(3-hydroxypropionate-co-3-hydroxybutyrate) accumulation in recombinant Escherichia coli.

Authors: H E Valentin; T A Mitsky; D A Mahadeo; M Tran; K J Gruys
Journal: Appl Environ Microbiol Date: 2000-12 Impact factor: 4.792

3. Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha.

Authors: Tina Lütke-Eversloh; Alexander Steinbüchel
Journal: FEMS Microbiol Lett Date: 2003-04-25 Impact factor: 2.742

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Identification of a new class of biopolymer: bacterial synthesis of a sulfur-containing polymer with thioester linkages.

Authors: T Lütke-Eversloh; K Bergander; H Luftmann; A Steinbüchel
Journal: Microbiology Date: 2001-01 Impact factor: 2.777

6. Production of 1,3-propanediol by Clostridium butyricum VPI 3266 using a synthetic medium and raw glycerol.

Authors: M González-Pajuelo; J C Andrade; I Vasconcelos
Journal: J Ind Microbiol Biotechnol Date: 2004-09-16 Impact factor: 3.346

7. Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli.

Authors: Xueming Tang; Yongsong Tan; Hong Zhu; Kai Zhao; Wei Shen
Journal: Appl Environ Microbiol Date: 2009-01-09 Impact factor: 4.792

8. Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products.

Authors: Syed Shams Yazdani; Ramon Gonzalez
Journal: Metab Eng Date: 2008-09-09 Impact factor: 9.783

9. Establishment of a novel anabolism-based addiction system with an artificially introduced mevalonate pathway: complete stabilization of plasmids as universal application in white biotechnology.

Authors: Jens Kroll; Anna Steinle; Rudolf Reichelt; Christian Ewering; Alexander Steinbüchel
Journal: Metab Eng Date: 2009-02-05 Impact factor: 9.783

10. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds.

Authors: P Spiekermann; B H Rehm; R Kalscheuer; D Baumeister; A Steinbüchel
Journal: Arch Microbiol Date: 1999-01 Impact factor: 2.552

21 in total

1. Heterologous Expression of Aldehyde Dehydrogenase from Saccharomyces cerevisiae in Klebsiella pneumoniae for 3-Hydroxypropionic Acid Production from Glycerol.

Authors: Kang Wang; Xi Wang; Xizhen Ge; Pingfang Tian
Journal: Indian J Microbiol Date: 2012-06-20 Impact factor: 2.461

Review 2. Microbial utilization of crude glycerol for the production of value-added products.

Authors: Rosemary Dobson; Vincent Gray; Karl Rumbold
Journal: J Ind Microbiol Biotechnol Date: 2011-09-25 Impact factor: 3.346

Review 3. Poly(3-hydroxypropionate): a promising alternative to fossil fuel-based materials.

Authors: Björn Andreessen; Nicolas Taylor; Alexander Steinbüchel
Journal: Appl Environ Microbiol Date: 2014-08-22 Impact factor: 4.792

4. Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B12.

Authors: Xinjun Feng; Mo Xian; Wei Liu; Chao Xu; Haibo Zhang; Guang Zhao
Journal: Bioengineered Date: 2015 Impact factor: 3.269

10. Optimization of the Expression of Genes Encoding Poly (3-hydroxyalkanoate) Synthase from Pseudomonas aeruginosa PTCC 1310 in Escherichia coli.

Authors: Daryoush Abedi; Maryam Beheshti; Ali Jahanian Najafabadi; Hamid Mir Mohammad Sadeghi; Vajihe Akbari
Journal: Avicenna J Med Biotechnol Date: 2012-01