BACKGROUND: Adult stem cells are promising novel therapies in regenerative medicine. We investigated effects of bone marrow-derived mesenchymal stem cells (MSCs) on intestinal mucosal permeability impaired by ischemia/reperfusion (I/R). METHODS: We used a common I/R model in rats to induce intestinal injury by clamping and unclamping the superior mesenteric artery (SMA) in female Sprague-Dawley rats. MSCs were directly injected into the small intestinal submucosa of the syngenic female rats. Control group were injected with the same volume of 0.9% sodium chloride. Small intestine samples were examined for the engraftment of donor-derived mesenchymal stem cells (MSCs) by Y chromosome in situ hybridization analysis. The small intestinal permeability and histomorphologic alternations were measured to evaluate the therapeutic effect of MSCs transplantation. RESULTS: Small intestinal permeability and villi injuries were significantly reduced in the MSCs administrated group compared with control group. MSCs administration accelerated the recovery of the intestinal barrier dysfunction. CONCLUSION: We concluded that submucosal infusion of MSCs might exert protective effects on the integrity of intestinal barrier.
BACKGROUND: Adult stem cells are promising novel therapies in regenerative medicine. We investigated effects of bone marrow-derived mesenchymal stem cells (MSCs) on intestinal mucosal permeability impaired by ischemia/reperfusion (I/R). METHODS: We used a common I/R model in rats to induce intestinal injury by clamping and unclamping the superior mesenteric artery (SMA) in female Sprague-Dawley rats. MSCs were directly injected into the small intestinal submucosa of the syngenic female rats. Control group were injected with the same volume of 0.9% sodium chloride. Small intestine samples were examined for the engraftment of donor-derived mesenchymal stem cells (MSCs) by Y chromosome in situ hybridization analysis. The small intestinal permeability and histomorphologic alternations were measured to evaluate the therapeutic effect of MSCs transplantation. RESULTS: Small intestinal permeability and villi injuries were significantly reduced in the MSCs administrated group compared with control group. MSCs administration accelerated the recovery of the intestinal barrier dysfunction. CONCLUSION: We concluded that submucosal infusion of MSCs might exert protective effects on the integrity of intestinal barrier.
Authors: Tao Sun; Guang-Zhou Gao; Rong-Fu Li; Xin Li; Da-Wei Li; Shan-Shan Wu; Anthony Et Yeo; Bo Jin Journal: Am J Transl Res Date: 2015-05-15 Impact factor: 4.060