Telomerase activity in peripheral blood mononuclear cells from HIV and HIV-HCV coinfected patients.

Replicative senescence of peripheral blood mononuclear cells (PBMC) plays an important role in the pathophysiology of chronic viral infections. Although there are controversial reports concerning telomerase activity in HIV monoinfected subjects, no data on HIV-HCV coinfected individuals is available. In this cross-sectional study telomerase activity was quantified in non-stimulated and mitogen-stimulated PBMC lysates from HIV-1 monoinfected and HIV-HCV coinfected individuals using real-time PCR. Up-regulation of telomerase activity after mitogen stimulation was impaired in PBMC of HIV monoinfected and HIV-HCV coinfected patients. The lack of an appropriate induction of this enzymatic activity after stimulus could partly account for immunosuppressive conditions observed in such patients. (c) 2009 Elsevier B.V. All rights reserved.