Literature DB >> 19932072

Identifying competitive protein antagonists for F-actin with reverse-phase high-performance liquid chromatography.

Jeffrey W Brown1, C James McKnight.   

Abstract

F-actin binding constants are traditionally determined by centrifugal cosedimentation with actin microfilaments, where bound protein is separated from actin with SDS-PAGE and quantitated using densitometry. Here, we demonstrate that UV quantitation of reverse-phase HPLC-separated proteins provides increased accuracy and sensitivity, can be fully automated, and allows one to perform F-actin competition assays on similar sized proteins. Copyright 2009 Elsevier Inc. All rights reserved.

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Year:  2009        PMID： 19932072      PMCID： PMC2812587          DOI： 10.1016/j.ab.2009.11.023

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

1.  Villin-type headpiece domains show a wide range of F-actin-binding affinities.

Authors:  D Vardar; A H Chishti; B S Frank; E J Luna; A A Noegel; S W Oh; M Schleicher; C J McKnight
Journal:  Cell Motil Cytoskeleton       Date:  2002-05

2.  Influence of ionic strength, actin state, and caldesmon construct size on the number of actin monomers in a caldesmon binding site.

Authors:  Scott Fredricksen; Anmei Cai; Boris Gafurov; Andrea Resetar; Joseph M Chalovich
Journal:  Biochemistry       Date:  2003-05-27       Impact factor: 3.162

3.  An actin-binding site containing a conserved motif of charged amino acid residues is essential for the morphogenic effect of villin.

Authors:  E Friederich; K Vancompernolle; C Huet; M Goethals; J Finidori; J Vandekerckhove; D Louvard
Journal:  Cell       Date:  1992-07-10       Impact factor: 41.582

Review 4.  Equilibrium binding of proteins to F-actin.

Authors:  Joseph M Chalovich
Journal:  Methods Mol Biol       Date:  2007

Review 5.  F-actin-binding proteins.

Authors:  A McGough
Journal:  Curr Opin Struct Biol       Date:  1998-04       Impact factor: 6.809

6.  Distinct regions of troponin I regulate Ca2+-dependent activation and Ca2+ sensitivity of the acto-S1-TM ATPase activity of the thin filament.

Authors:  J E Van Eyk; L T Thomas; B Tripet; R J Wiesner; J R Pearlstone; C S Farah; F C Reinach; R S Hodges
Journal:  J Biol Chem       Date:  1997-04-18       Impact factor: 5.157

7.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

8.  Interpretation of binding curves obtained with high receptor concentrations: practical aid for computer analysis.

Authors:  S Swillens
Journal:  Mol Pharmacol       Date:  1995-06       Impact factor: 4.436

9.  Cooperative binding of myosin subfragment-1 to the actin-troponin-tropomyosin complex.

Authors:  L E Greene; E Eisenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

Review 10.  Actin binding proteins: regulation of cytoskeletal microfilaments.

Authors:  C G dos Remedios; D Chhabra; M Kekic; I V Dedova; M Tsubakihara; D A Berry; N J Nosworthy
Journal:  Physiol Rev       Date:  2003-04       Impact factor: 37.312
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  3 in total

1.  Competition between intradomain and interdomain interactions: a buried salt bridge is essential for villin headpiece folding and actin binding.

Authors:  Laura E Packer; Benben Song; Daniel P Raleigh; C James McKnight
Journal:  Biochemistry       Date:  2011-04-15       Impact factor: 3.162

2.  On unsatisfied hydrogen bonds in the N-terminal subdomain of villin headpiece.

Authors:  Jeffrey W Brown; Jeremiah D Farelli; C James McKnight
Journal:  J Mol Biol       Date:  2011-08-31       Impact factor: 5.469

3.  The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9).

Authors:  Lin Chen; Jeffrey W Brown; Yee-Foong Mok; Danny M Hatters; C James McKnight
Journal:  J Biol Chem       Date:  2013-01-25       Impact factor: 5.157
  3 in total

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