OBJECTIVE: The aim of this study was to evaluate the composition of the biofilm accumulated around ligatures in rats by checkerboard DNA-DNA hybridization using probes made from human oral bacterial species. METHODS: 12 Wistar rats were selected for this study. One of the mandibular first molars of each animal received a ligature, while the contralateral tooth was left unligated to be used as a control. Forty-two days later, the ligatures and one sample of biofilm from each unligated teeth were analyzed by checkerboard DNA-DNA hybridization for 40 human periodontal species. The area of bone loss in the furcation area was determined histometrically. RESULTS: The unligated teeth did not present any of the bacterial species tested. Twenty five species were detected in the ligatures. Streptococcus- and Actinomyces-like species were found in high mean counts, followed by Fusobacterium-, Prevotella nigrescens- and Parvimonas micra-like species and Porphyromonas gingivalis- and Aggregatibacter actinomycetemcomitans-like species. Greater bone loss was observed in the furcation area of the ligated than of the unligated group (p<0.0001). CONCLUSION: At 42 days, the ligature biofilm in rats presents various bacterial species that hybridize to probes of periodontal bacterial species commonly observed in human. Copyright 2009 Elsevier Ltd. All rights reserved.
OBJECTIVE: The aim of this study was to evaluate the composition of the biofilm accumulated around ligatures in rats by checkerboard DNA-DNA hybridization using probes made from human oral bacterial species. METHODS: 12 Wistar rats were selected for this study. One of the mandibular first molars of each animal received a ligature, while the contralateral tooth was left unligated to be used as a control. Forty-two days later, the ligatures and one sample of biofilm from each unligated teeth were analyzed by checkerboard DNA-DNA hybridization for 40 human periodontal species. The area of bone loss in the furcation area was determined histometrically. RESULTS: The unligated teeth did not present any of the bacterial species tested. Twenty five species were detected in the ligatures. Streptococcus- and Actinomyces-like species were found in high mean counts, followed by Fusobacterium-, Prevotella nigrescens- and Parvimonas micra-like species and Porphyromonas gingivalis- and Aggregatibacter actinomycetemcomitans-like species. Greater bone loss was observed in the furcation area of the ligated than of the unligated group (p<0.0001). CONCLUSION: At 42 days, the ligature biofilm in rats presents various bacterial species that hybridize to probes of periodontal bacterial species commonly observed in human. Copyright 2009 Elsevier Ltd. All rights reserved.
Authors: Marcelo O Freire; Parish P Sedghizadeh; Christoph Schaudinn; Amita Gorur; Jennifer S Downey; Jeong-Ho Choi; Weizhen Chen; Joong-Ki Kook; Casey Chen; Steven D Goodman; Homayoun H Zadeh Journal: J Periodontol Date: 2011-01-11 Impact factor: 6.993
Authors: M O Freire; A Devaraj; A Young; J B Navarro; J S Downey; C Chen; L O Bakaletz; H H Zadeh; S D Goodman Journal: Mol Oral Microbiol Date: 2016-04-05 Impact factor: 3.563