| Literature DB >> 1993071 |
T Sawamura1, Y Kasuya, Y Matsushita, N Suzuki, O Shinmi, N Kishi, Y Sugita, M Yanagisawa, K Goto, T Masaki.
Abstract
Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increase that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1: big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly.Entities:
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Year: 1991 PMID： 1993071 DOI： 10.1016/0006-291x(91)91485-u
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575