Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors. III. dCOP-diacylglycerol formation in tonsillar lymphocytes.

In addition to the water-soluble deoxyliponucleotides (Spasokukotskaja et al. (1988), Biochem. Biophys. Res. Commun. 155, 923), a lipid compound was shown to be labeled from external 3H-deoxycytidine (5-3H-CdR) in infant tonsillar lymphocytes. Chlorpromazine enhanced the labeling of this compound, identified by TLC as 3H-dCDP-diacylglycerol (3H-dCOP-DAG). The deoxynucleotide salvage pathway seems to be the main source for dCDP-DAG synthesis, as hydroxyurea increased its labeling from CdR. myo-Inositol induced the disappearance of 5-3H-dCOP-DAG, suggesting its utilization for phosphatidylinositol synthesis. 3H-Arabinosyl-Cytosine (araC) is also incorporated into the lipidic fraction at a rate comparable to its incorporation into DNA, supporting the effect of araC on membrane functions.