BACKGROUND: Dengue is the most prevalent mosquito-borne viral disease in humans and a major urban public health problem worldwide. METHODS: A prospective cohort study of approximately 3800 children initially aged 2-9 years was established in Managua, Nicaragua, in 2004 to study the natural history of dengue transmission in an urban pediatric population. Blood samples from healthy subjects were collected annually prior to the dengue season, and identification of dengue cases occurred via enhanced passive surveillance at the study health center. RESULTS: Over the first four years of the study, seroprevalence of anti-dengue virus (DENV) antibodies increased from 22%-40% in the 2-year-old cohort and 90%-95% in the 9-year-old cohort. The incidence of symptomatic dengue cases and the ratio of inapparent to symptomatic DENV infection varied substantially from year to year. The switch in dominant transmission from DENV-1 to DENV-2 was accompanied by an increase in disease severity but, paradoxically, a decrease in transmission. Phylogeographic analysis of full-length DENV-2 sequences revealed strong geographic clustering of dengue cases. CONCLUSIONS: This large-scale cohort study of dengue in the Americas demonstrates year-to-year variation of dengue within a pediatric population, revealing expected patterns in transmission while highlighting the impact of interventions, climate, and viral evolution.
BACKGROUND: Dengue is the most prevalent mosquito-borne viral disease in humans and a major urban public health problem worldwide. METHODS: A prospective cohort study of approximately 3800 children initially aged 2-9 years was established in Managua, Nicaragua, in 2004 to study the natural history of dengue transmission in an urban pediatric population. Blood samples from healthy subjects were collected annually prior to the dengue season, and identification of dengue cases occurred via enhanced passive surveillance at the study health center. RESULTS: Over the first four years of the study, seroprevalence of anti-dengue virus (DENV) antibodies increased from 22%-40% in the 2-year-old cohort and 90%-95% in the 9-year-old cohort. The incidence of symptomatic dengue cases and the ratio of inapparent to symptomatic DENV infection varied substantially from year to year. The switch in dominant transmission from DENV-1 to DENV-2 was accompanied by an increase in disease severity but, paradoxically, a decrease in transmission. Phylogeographic analysis of full-length DENV-2 sequences revealed strong geographic clustering of dengue cases. CONCLUSIONS: This large-scale cohort study of dengue in the Americas demonstrates year-to-year variation of dengue within a pediatric population, revealing expected patterns in transmission while highlighting the impact of interventions, climate, and viral evolution.
Authors: Kevin R Porter; Charmagne G Beckett; Herman Kosasih; Ratna Irsiana Tan; Bachti Alisjahbana; Pandji Irani Fianza Rudiman; Susana Widjaja; Erlin Listiyaningsih; Chairin Nisa Ma'Roef; James L McArdle; Ida Parwati; Primal Sudjana; Hadi Jusuf; Djoko Yuwono; Suharyono Wuryadi Journal: Am J Trop Med Hyg Date: 2005-01 Impact factor: 2.345
Authors: R R Graham; M Juffrie; R Tan; C G Hayes; I Laksono; C Ma'roef; K R Porter; S B Halstead Journal: Am J Trop Med Hyg Date: 1999-09 Impact factor: 2.345
Authors: G Kouri; M Valdéz; L Arguello; M G Guzmán; L Valdés; M Soler; J Bravo Journal: Rev Inst Med Trop Sao Paulo Date: 1991 Sep-Oct Impact factor: 1.846
Authors: Henrik Salje; Justin Lessler; Timothy P Endy; Frank C Curriero; Robert V Gibbons; Ananda Nisalak; Suchitra Nimmannitya; Siripen Kalayanarooj; Richard G Jarman; Stephen J Thomas; Donald S Burke; Derek A T Cummings Journal: Proc Natl Acad Sci U S A Date: 2012-05-29 Impact factor: 11.205
Authors: Poornima Parameswaran; Patrick Charlebois; Yolanda Tellez; Andrea Nunez; Elizabeth M Ryan; Christine M Malboeuf; Joshua Z Levin; Niall J Lennon; Angel Balmaseda; Eva Harris; Matthew R Henn Journal: J Virol Date: 2012-05-30 Impact factor: 5.103
Authors: Leah C Katzelnick; Magelda Montoya; Lionel Gresh; Angel Balmaseda; Eva Harris Journal: Proc Natl Acad Sci U S A Date: 2016-01-04 Impact factor: 11.205
Authors: In-Kyu Yoon; Anon Srikiatkhachorn; Laura Hermann; Darunee Buddhari; Thomas W Scott; Richard G Jarman; Jared Aldstadt; Ananda Nisalak; Suwich Thammapalo; Piraya Bhoomiboonchoo; Mammen P Mammen; Sharone Green; Robert V Gibbons; Timothy P Endy; Alan L Rothman Journal: Am J Trop Med Hyg Date: 2013-10-14 Impact factor: 2.345