| Literature DB >> 19928994 |
Guoxin Jiang1, Andrei S Susha, Andrey A Lutich, Fernando D Stefani, Jochen Feldmann, Andrey L Rogach.
Abstract
Electrostatic complexes of a water-soluble fluorescent conjugated polymer, poly[9,9-bis(3'-((N,N-dimethyl)-N-ethylammonium)propyl)-2,7-fluorene-alt-1,4-phenylene]dibromide (PDFD), and water-soluble CdTe quantum dots (QDs) are designed to provide a cascaded FRET for DNA hybridization detection. PDFD has two functions in the detection scheme: as a light-harvesting antenna, it enhances the emission of QDs by the first level FRET and inverts the sign of the surface charge of QDs, thus providing a positively charged surface to allow negatively charged dye-labeled DNA to interact with the resulting complex. This interaction causes the second level FRET to infrared-emitting dye labeled on the probe DNA, providing a reliable signal-on sensing platform discriminating between complementary and non-complementary DNA. A detailed spectroscopic study offers a clear description of photophysical processes in the designed polymer/QD/DNA complex, providing ample potential for further sensitivity and selectivity improvements.Entities:
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Year: 2009 PMID： 19928994 DOI： 10.1021/nn901324y
Source DB: PubMed Journal: ACS Nano ISSN： 1936-0851 Impact factor: 15.881