| Literature DB >> 19924480 |
Kimberly Musa Specht1, Kyle H Sheetz, Courtney M Alexander, Lilian T Lamech, Lauren H O'Connor, Dawn M Walker, Hilary P Stevenson.
Abstract
Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli. The fusion proteins were isolated and shown to bind beta-lactams, indicating these putative PBPs have penicillin-binding activity.Entities:
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Year: 2009 PMID： 19924480 DOI： 10.1007/s00284-009-9537-1
Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188