Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase.

Literature DB >> 19923749

Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase.

Lakshmi Dharmarajan¹, Jessica L Kraszewski, Biswarup Mukhopadhyay, Pete W Dunten.

Abstract

An archaeal-type phosphoenolpyruvate carboxylase (PepcA) from Clostridium perfringens has been expressed in Escherichia coli in a soluble form with an amino-terminal His tag. The recombinant protein is enzymatically active and two crystal forms have been obtained. Complete diffraction data extending to 3.13 angstrom resolution have been measured from a crystal soaked in KAu(CN)(2), using radiation at a wavelength just above the Au L(III) edge. The asymmetric unit contains two tetramers of PepcA.

Entities: Disease Species

Mesh：

Substances：

Year: 2009 PMID： 19923749 PMCID： PMC2777057 DOI： 10.1107/S1744309109042663

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

17 in total

1. Crystal structures of C4 form maize and quaternary complex of E. coli phosphoenolpyruvate carboxylases.

Authors: Hiroyoshi Matsumura; Yong Xie; Shunsuke Shirakata; Tsuyoshi Inoue; Takeo Yoshinaga; Yoshihisa Ueno; Katsura Izui; Yasushi Kai
Journal: Structure Date: 2002-12 Impact factor: 5.006

Review 2. Phosphoenolpyruvate carboxylase: a new era of structural biology.

Authors: Katsura Izui; Hiroyoshi Matsumura; Tsuyoshi Furumoto; Yasushi Kai
Journal: Annu Rev Plant Biol Date: 2004 Impact factor: 26.379

3. A short history of SHELX.

Authors: George M Sheldrick
Journal: Acta Crystallogr A Date: 2007-12-21 Impact factor: 2.290

4. A novel mechanism of allosteric regulation of archaeal phosphoenolpyruvate carboxylase: a combined approach to structure-based alignment and model assessment.

Authors: Hiroyoshi Matsumura; Katsura Izui; Kenji Mizuguchi
Journal: Protein Eng Des Sel Date: 2006-06-30 Impact factor: 1.650

Review 5. Overexpression of C(4)-cycle enzymes in transgenic C(3) plants: a biotechnological approach to improve C(3)-photosynthesis.

Authors: Rainer E Häusler; Heinz-Josef Hirsch; Fritz Kreuzaler; Christoph Peterhänsel
Journal: J Exp Bot Date: 2002-04 Impact factor: 6.992

6. Activity regulation and physiological impacts of maize C(4)-specific phosphoenolpyruvate carboxylase overproduced in transgenic rice plants.

Authors: Hiroshi Fukayama; Marshall D Hatch; Tesshu Tamai; Hiroko Tsuchida; Sizue Sudoh; Robert T Furbank; Mitsue Miyao
Journal: Photosynth Res Date: 2003 Impact factor: 3.573

Review 7. Transgenic approaches to manipulate the environmental responses of the C3 carbon fixation cycle.

Authors: Christine A Raines
Journal: Plant Cell Environ Date: 2006-03 Impact factor: 7.228

8. Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism.

Authors: Thijs J G Ettema; Kira S Makarova; Gera L Jellema; Hinco J Gierman; Eugene V Koonin; Martijn A Huynen; Willem M de Vos; John van der Oost
Journal: J Bacteriol Date: 2004-11 Impact factor: 3.490