OBJECTIVE AND DESIGN: In this study we investigated the role of histamine on the extramedullary hematopoiesis. METHODS: Male histidine decarboxylase knockout (HDC(-/-)) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student's t test, ANOVA, and Holm-Sidak post-hoc test were used. RESULTS: By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC(-/-) mice (P < 0.01). The proliferative capacity and interleukin-3 signaling of stem cells were impaired in HDC(-/-) mice. STAT5 mRNA expression was decreased in granulocyte-myeloid colonies by 72.9 +/- 8.6% (P < 0.001), compared to the wild-type. CONCLUSIONS: The absence of histamine adversely affects splenic hematopoiesis via direct and indirect mechanisms.
OBJECTIVE AND DESIGN: In this study we investigated the role of histamine on the extramedullary hematopoiesis. METHODS: Male histidine decarboxylase knockout (HDC(-/-)) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student's t test, ANOVA, and Holm-Sidak post-hoc test were used. RESULTS: By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC(-/-)mice (P < 0.01). The proliferative capacity and interleukin-3 signaling of stem cells were impaired in HDC(-/-)mice. STAT5 mRNA expression was decreased in granulocyte-myeloid colonies by 72.9 +/- 8.6% (P < 0.001), compared to the wild-type. CONCLUSIONS: The absence of histamine adversely affects splenic hematopoiesis via direct and indirect mechanisms.
Authors: Wolfgang Wagner; Alexandra Ansorge; Ute Wirkner; Volker Eckstein; Christian Schwager; Jonathon Blake; Katrin Miesala; Jan Selig; Rainer Saffrich; Wilhelm Ansorge; Anthony D Ho Journal: Blood Date: 2004-04-13 Impact factor: 22.113
Authors: S Teglund; C McKay; E Schuetz; J M van Deursen; D Stravopodis; D Wang; M Brown; S Bodner; G Grosveld; J N Ihle Journal: Cell Date: 1998-05-29 Impact factor: 41.582