PURPOSE: To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress. METHODS: In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41 degrees C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41 degrees C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days. RESULTS: In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5 degrees C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups. CONCLUSION: The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.
PURPOSE: To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress. METHODS: In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41 degrees C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41 degrees C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days. RESULTS: In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5 degrees C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups. CONCLUSION: The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.
Authors: Jessica L Klabnik; Lane K Christenson; Sumedha S A Gunewardena; Ky G Pohler; Louisa A Rispoli; Rebecca R Payton; Sarah E Moorey; F Neal Schrick; J Lannett Edwards Journal: J Anim Sci Date: 2022-07-01 Impact factor: 3.338
Authors: Julia R Rowinski; Louisa A Rispoli; Rebecca R Payton; Liesel G Schneider; F Neal Schrick; Kyle J McLean; J Lannett Edwards Journal: Anim Reprod Date: 2021-02-16 Impact factor: 1.807
Authors: Kelly A Campen; Chelsea R Abbott; Louisa A Rispoli; Rebecca R Payton; Arnold M Saxton; J Lannett Edwards Journal: J Reprod Dev Date: 2018-06-25 Impact factor: 2.214
Authors: Louisa A Rispoli; J Lannett Edwards; Ky G Pohler; Stephen Russell; Richard I Somiari; Rebecca R Payton; F Neal Schrick Journal: PLoS One Date: 2019-12-30 Impact factor: 3.240