Literature DB >> 1991515

In vivo promoter activity of the synthetic Pribnow box.

O N Koroleva1, V L Drutsa.   

Abstract

The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA)1-4, containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been shown that these inserts are able to induce an efficient in vivo expression of the gal operon.

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Year:  1991        PMID： 1991515     DOI： 10.1016/0014-5793(91)80118-m

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  3 in total

1.  An assessment of neural network and statistical approaches for prediction of E. coli promoter sites.

Authors:  P B Horton; M Kanehisa
Journal:  Nucleic Acids Res       Date:  1992-08-25       Impact factor: 16.971

2.  Use of oligonucleotides and nick translation for site-directed mutagenesis in plasmids.

Authors:  V L Drutsa; V R Kaberdin
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

3.  Effects of substitutions at position 180 in the Escherichia coli RNA polymerase σ 70 subunit.

Authors:  Olga N Koroleva; Stephen J W Busby; Valeriy L Drutsa
Journal:  J Biosci       Date:  2011-03       Impact factor: 1.826
  3 in total

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