AIMS: The chemokine receptor CXCR4 plays a prominent role in the biology of many different tumours, promoting angiogenesis and metastasis. The impact of CXCR4 expression on tumour biology has been described in various gastrointestinal malignancies, but data on its in situ expression and clinicopathological correlations are sparse. Using a novel specific rabbit anti-CXCR4 antibody, the aim was to assess CXCR4 expression immunohistochemically on tissue microarrays generated from 402 colorectal cancers (CRCs) and compare it with CXCL12 expression and various clinicopathological parameters. METHODS AND RESULTS: CXCR4-expressing tumour cells were observed in 31% of the cases, and expression correlated only with blood vessel invasion (P = 0.049). Furthermore, CXCR4 was found in tumour microvessels in 25% of CRCs. This pattern of CXCR4 expression correlated significantly with T- (P = 0.008), N- (P = 0.009), M- (P = 0.043), L- (P = 0.014) and V-category (P = 0.043) as well as with International Union Against Cancer (UICC) stage (P = 0.001). Furthermore, in node negative CRCs, vascular CXCR4 expression was an independent adverse prognostic factor [hazard ratio 2.87 (1.31-6.29), P = 0.009]. No correlation with CXCL12 expression was found. CONCLUSIONS: Our data provide evidence that CXCR4 plays an important role in tumour angiogenesis of CRC. Therefore, the CXCR4 pathway is a promising therapeutic target for anti-angiogenic therapies in CRC.
AIMS: The chemokine receptor CXCR4 plays a prominent role in the biology of many different tumours, promoting angiogenesis and metastasis. The impact of CXCR4 expression on tumour biology has been described in various gastrointestinal malignancies, but data on its in situ expression and clinicopathological correlations are sparse. Using a novel specific rabbit anti-CXCR4 antibody, the aim was to assess CXCR4 expression immunohistochemically on tissue microarrays generated from 402 colorectal cancers (CRCs) and compare it with CXCL12 expression and various clinicopathological parameters. METHODS AND RESULTS:CXCR4-expressing tumour cells were observed in 31% of the cases, and expression correlated only with blood vessel invasion (P = 0.049). Furthermore, CXCR4 was found in tumour microvessels in 25% of CRCs. This pattern of CXCR4 expression correlated significantly with T- (P = 0.008), N- (P = 0.009), M- (P = 0.043), L- (P = 0.014) and V-category (P = 0.043) as well as with International Union Against Cancer (UICC) stage (P = 0.001). Furthermore, in node negative CRCs, vascular CXCR4 expression was an independent adverse prognostic factor [hazard ratio 2.87 (1.31-6.29), P = 0.009]. No correlation with CXCL12 expression was found. CONCLUSIONS: Our data provide evidence that CXCR4 plays an important role in tumour angiogenesis of CRC. Therefore, the CXCR4 pathway is a promising therapeutic target for anti-angiogenic therapies in CRC.
Authors: Barbara Ingold; Eva Simon; Ute Ungethüm; Ralf-Jürgen Kuban; Berit M Müller; Amelie Lupp; Ulf Neumann; Matthias P A Ebert; Carsten Denkert; Wilko Weichert; Stefan Schulz; Christoph Röcken Journal: PLoS One Date: 2010-04-08 Impact factor: 3.240
Authors: Claudia Rubie; Vilma O Frick; Pirus Ghadjar; Mathias Wagner; Christoph Justinger; Sabrina K Faust; Benjamin Vicinus; Stefan Gräber; Otto Kollmar; Martin K Schilling Journal: J Transl Med Date: 2011-02-24 Impact factor: 5.531
Authors: Marta Braga; Chee Hau Leow; Javier Hernandez Gil; Jin H Teh; Laurence Carroll; Nicholas J Long; Meng-Xing Tang; Eric O Aboagye Journal: PLoS One Date: 2021-11-18 Impact factor: 3.240