BACKGROUND: Antigen detection, which has proven useful in diagnosis of disseminated histoplasmosis, has not been studied in acute pulmonary histoplasmosis (APH). Because treatment is indicated in most patients with moderately severe or severe APH, antigen detection for rapid diagnosis could be helpful. METHODS: Histoplasma antigen detection was evaluated in 130 patients with APH. RESULTS: Antigenuria was detected in 64.6%, antigenemia in 68.6%, and antibody in 64.3%. If both urine and serum specimens were tested, antigen was detected in 82.8%, of which 45.8% had antigenemia only; and if both antigen and antibody were measured, results were positive in 93.3%, of which antigen only was positive in 35.7%. CONCLUSIONS: Testing for antigenemia, antigenuria, and antibodies using the complement fixation test offers a sensitive, noninvasive method for diagnosis of APH.
BACKGROUND: Antigen detection, which has proven useful in diagnosis of disseminated histoplasmosis, has not been studied in acute pulmonary histoplasmosis (APH). Because treatment is indicated in most patients with moderately severe or severe APH, antigen detection for rapid diagnosis could be helpful. METHODS: Histoplasma antigen detection was evaluated in 130 patients with APH. RESULTS: Antigenuria was detected in 64.6%, antigenemia in 68.6%, and antibody in 64.3%. If both urine and serum specimens were tested, antigen was detected in 82.8%, of which 45.8% had antigenemia only; and if both antigen and antibody were measured, results were positive in 93.3%, of which antigen only was positive in 35.7%. CONCLUSIONS: Testing for antigenemia, antigenuria, and antibodies using the complement fixation test offers a sensitive, noninvasive method for diagnosis of APH.
Authors: Maha Assi; Stanley Martin; L Joseph Wheat; Chadi Hage; Alison Freifeld; Robin Avery; John W Baddley; Paschalis Vergidis; Rachel Miller; David Andes; Jo-Anne H Young; Kassem Hammoud; Shirish Huprikar; David McKinsey; Thein Myint; Julia Garcia-Diaz; Eden Esguerra; E J Kwak; Michele Morris; Kathleen M Mullane; Vidhya Prakash; Steven D Burdette; Mohammad Sandid; Jana Dickter; Darin Ostrander; Smyrna Abou Antoun; Daniel R Kaul Journal: Clin Infect Dis Date: 2013-09-17 Impact factor: 9.079
Authors: Patricia Connolly; Chadi A Hage; J Ryan Bariola; Eric Bensadoun; Mark Rodgers; Robert W Bradsher; L Joseph Wheat Journal: Clin Vaccine Immunol Date: 2011-11-23
Authors: Sean X Zhang; N Esther Babady; Kimberly E Hanson; Amanda T Harrington; Paige M K Larkin; Sixto M Leal; Paul M Luethy; Isabella W Martin; Preeti Pancholi; Gary W Procop; Stefan Riedel; Seyedmojtaba Seyedmousavi; Kaede V Sullivan; Thomas J Walsh; Shawn R Lockhart Journal: J Clin Microbiol Date: 2021-06-18 Impact factor: 5.948