Literature DB >> 19907997

Study of epidermal differentiation in human keratinocytes cultured in autocrine conditions.

Frédéric Minner1, Françoise Herphelin, Yves Poumay.   

Abstract

This chapter deals with protocols to set up human keratinocyte cultures in serum-free conditions and lead them to autocrine autonomously growing conditions. These conditions have proven adequate for studies of epidermal differentiation by measurements of the expression of typical early and late differentiation markers. The chapter also deals with the use of quantitative RT-PCR in order to determine the epidermal marker gene expression levels by comparison with adequate housekeeping genes.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 19907997     DOI： 10.1007/978-1-60761-380-0_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Hyaluronan Does Not Regulate Human Epidermal Keratinocyte Proliferation and Differentiation.

Authors:  Jérémy Malaisse; Valérie Pendaries; Fanny Hontoir; Valérie De Glas; Daniel Van Vlaender; Michel Simon; Catherine Lambert de Rouvroit; Yves Poumay; Bruno Flamion
Journal:  J Biol Chem       Date:  2015-12-01       Impact factor: 5.157

2.  Krüppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes.

Authors:  Florian Spörl; Sandra Korge; Karsten Jürchott; Minetta Wunderskirchner; Katja Schellenberg; Sven Heins; Aljona Specht; Claudia Stoll; Roman Klemz; Bert Maier; Horst Wenck; Annika Schrader; Dieter Kunz; Thomas Blatt; Achim Kramer
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-18       Impact factor: 11.205

3.  Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.

Authors:  Alena Rajnochová Svobodová; Alena Ryšavá; Kateřina Čížková; Lenka Roubalová; Jitka Ulrichová; Jiří Vrba; Bohumil Zálešák; Jitka Vostálová
Journal:  Photochem Photobiol Sci       Date:  2021-11-27       Impact factor: 3.982

4.  Preclinical assessment of dual CYP26[A1/B1] inhibitor, DX308, as an improved treatment for keratinization disorders.

Authors:  J G S Veit; Y Poumay; D Mendes; J Kreitinger; L Walker; A Paquet; C Menigot; F Zolezzi; A S Paller; P Diaz
Journal:  Skin Health Dis       Date:  2021-03-26

Review 5.  Characterization of CYP26B1-Selective Inhibitor, DX314, as a Potential Therapeutic for Keratinization Disorders.

Authors:  Joachim G S Veit; Valérie De Glas; Benoît Balau; Haoming Liu; Florence Bourlond; Amy S Paller; Yves Poumay; Philippe Diaz
Journal:  J Invest Dermatol       Date:  2020-06-04       Impact factor: 8.551

6.  TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation.

Authors:  Aurélie Hayez; Edith Roegiers; Jérémy Malaisse; Benoit Balau; Christiane Sterpin; Younes Achouri; Catherine Lambert De Rouvroit; Yves Poumay; Carine Michiels; Olivier De Backer
Journal:  PLoS One       Date:  2016-01-19       Impact factor: 3.240

7.  In Vitro AuNPs' Cytotoxicity and Their Effect on Wound Healing.

Authors:  Veronika Pivodová; Jana Franková; Adéla Galandáková; Jitka Ulrichová
Journal:  Nanobiomedicine (Rij)       Date:  2015-01-01

8.  Deletion of TNFAIP6 Gene in Human Keratinocytes Demonstrates a Role for TSG-6 to Retain Hyaluronan Inside Epidermis.

Authors:  Céline Evrard; Emilie Faway; Evelyne De Vuyst; Olivier Svensek; Valérie De Glas; David Bergerat; Michel Salmon; Olivier De Backer; Bruno Flamion; Hélène Le-Buanec; Catherine Lambert de Rouvroit; Yves Poumay
Journal:  JID Innov       Date:  2021-08-23

Review 9.  Modeling an Optimal 3D Skin-on-Chip within Microfluidic Devices for Pharmacological Studies.

Authors:  Estibaliz Fernandez-Carro; Maricke Angenent; Tamara Gracia-Cazaña; Yolanda Gilaberte; Clara Alcaine; Jesús Ciriza
Journal:  Pharmaceutics       Date:  2022-07-06       Impact factor: 6.525

10.  TMEM45A is essential for hypoxia-induced chemoresistance in breast and liver cancer cells.

Authors:  Lionel Flamant; Edith Roegiers; Michael Pierre; Aurélie Hayez; Christiane Sterpin; Olivier De Backer; Thierry Arnould; Yves Poumay; Carine Michiels
Journal:  BMC Cancer       Date:  2012-09-06       Impact factor: 4.430
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.