PURPOSE: To investigate the effects of a disintegrin and metalloproteinase 28(ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human periodontal ligament stem cell(HPDLSC) and possible mechanism. METHODS: HPDLSC was isolated, cultured in vitro and identified. Synthetic ADAM28 AS-ODN and S-ODN were transfected into HPDLSC, respectively. MTT chromatometry, enzyme dynamics and flow cytometry (FCM) were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HPDLSC. Statistical significance was assessed by the Student-Newman-Keuls (SNK) test with SPSS 13.0 software package. RESULTS: In ADAM28 AS-ODN group, the proliferation activity and index of HPDLSC were significantly lower than those of S-ODN group and untransfected group, alkaline phosphatase(ALP) secretion level and percentage of apoptotic cells went up obviously. Significant difference was detected between AS-ODN group and other groups (P<0.01). CONCLUSIONS: ADAM28 AS-ODN could inhibit HPDLSC proliferation and influence the changes of cell cycle, promote ALP secretion and induce HPDLSC apoptosis significantly.
PURPOSE: To investigate the effects of a disintegrin and metalloproteinase 28(ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human periodontal ligament stem cell(HPDLSC) and possible mechanism. METHODS: HPDLSC was isolated, cultured in vitro and identified. Synthetic ADAM28AS-ODN and S-ODN were transfected into HPDLSC, respectively. MTT chromatometry, enzyme dynamics and flow cytometry (FCM) were used to detect the effects of ADAM28AS-ODN on biological characteristics of HPDLSC. Statistical significance was assessed by the Student-Newman-Keuls (SNK) test with SPSS 13.0 software package. RESULTS: In ADAM28AS-ODN group, the proliferation activity and index of HPDLSC were significantly lower than those of S-ODN group and untransfected group, alkaline phosphatase(ALP) secretion level and percentage of apoptotic cells went up obviously. Significant difference was detected between AS-ODN group and other groups (P<0.01). CONCLUSIONS:ADAM28AS-ODN could inhibit HPDLSC proliferation and influence the changes of cell cycle, promote ALP secretion and induce HPDLSC apoptosis significantly.