| Literature DB >> 19904407 |
T Baier1, T E Hansen-Hagge, R Gransee, A Crombé, S Schmahl, C Paulus, K S Drese, H Keegan, C Martin, J J O'Leary, L Furuberg, L Solli, P Grønn, I M Falang, A Karlgård, A Gulliksen, F Karlsen.
Abstract
A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.Entities:
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Year: 2009 PMID： 19904407 DOI： 10.1039/b910421f
Source DB: PubMed Journal: Lab Chip ISSN： 1473-0189 Impact factor: 6.799