| Literature DB >> 19900401 |
Maria V Abramova1, Evgeny A Zatulovskiy, Svetlana B Svetlikova, Alexander N Kukushkin, Valery A Pospelov.
Abstract
HDAC inhibitors induce cell cycle arrest of E1A+Ras-transformed cells accompanied by e2f1 gene down-regulation and activation of Wnt pathway. Here we show that e2f1 expression is regulated through the Wnt/Tcf-pathway: e2f1 promoter activity is inhibited by sodium butyrate (NaB) and by overexpression of beta-catenin/Tcf. The e2f1 promoter was found to contain two putative Tcf-binding elements: the proximal one competes well with canonical Tcf element in DNA-binding assay. Being inserted into luciferase reporter vector, the identified element provides positive transcriptional regulation in response to beta-catenin/Tcf co-transfection and NaB treatment. Thus we have firstly demonstrated that e2f1 belongs to genes regulated through Wnt/beta-catenin/Tcf pathway. Copyright 2009 Elsevier Inc. All rights reserved.Entities:
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Year: 2009 PMID： 19900401 DOI： 10.1016/j.bbrc.2009.11.020
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575