PURPOSE: To determine how brain-derived neurotrophic factor (BDNF) protects photoreceptors against phototoxicity. METHODS: Iris pigment epithelial cells (IPE) that were transduced with different concentrations of adeno-associated virus (AAV) mediated BDNF (AAV-BDNF-IPE) were transplanted into the subretinal space of rats. We also injected small interfering RNAs (siRNAs) for TrkB, a BDNF receptor. The rats were exposed to continuous light to induce phototoxicity. We examined the expression of TrkB in the retina by Western blot and immunohistochemistry. RESULTS: Significant photoreceptor protection was detected when more than 1 x 10(7) capsids/ml AAV-BDNF was transplanted. An intravitreal injection of siRNAs showed that the photoreceptor protection by AAV-BDNF-IPE was reduced by injecting the siRNA of TrkB-T1, one of the TrkB isoforms. TrkB-T1 was slightly upregulated by Western blot, and one of the cells that upregulated TrkB-T1 was Muller cells by immunohistochemistry. CONCLUSION: We conclude that Muller cells are one of the cells responsible for the expression of TrkBs, and TrkB-T1 may play a role in the protection of photoreceptors against phototoxicity.
PURPOSE: To determine how brain-derived neurotrophic factor (BDNF) protects photoreceptors against phototoxicity. METHODS: Iris pigment epithelial cells (IPE) that were transduced with different concentrations of adeno-associated virus (AAV) mediated BDNF (AAV-BDNF-IPE) were transplanted into the subretinal space of rats. We also injected small interfering RNAs (siRNAs) for TrkB, a BDNF receptor. The rats were exposed to continuous light to induce phototoxicity. We examined the expression of TrkB in the retina by Western blot and immunohistochemistry. RESULTS: Significant photoreceptor protection was detected when more than 1 x 10(7) capsids/ml AAV-BDNF was transplanted. An intravitreal injection of siRNAs showed that the photoreceptor protection by AAV-BDNF-IPE was reduced by injecting the siRNA of TrkB-T1, one of the TrkB isoforms. TrkB-T1 was slightly upregulated by Western blot, and one of the cells that upregulated TrkB-T1 was Muller cells by immunohistochemistry. CONCLUSION: We conclude that Muller cells are one of the cells responsible for the expression of TrkBs, and TrkB-T1 may play a role in the protection of photoreceptors against phototoxicity.
Authors: Rachael S Allen; Adam M Hanif; Marissa A Gogniat; Brian C Prall; Raza Haider; Moe H Aung; Megan C Prunty; Lukas M Mees; Monica M Coulter; Cara T Motz; Jeffrey H Boatright; Machelle T Pardue Journal: Eur J Neurosci Date: 2018-04-03 Impact factor: 3.386
Authors: Adam M Hanif; Eric C Lawson; Megan Prunty; Marissa Gogniat; Moe H Aung; Ranjay Chakraborty; Jeffrey H Boatright; Machelle T Pardue Journal: Invest Ophthalmol Vis Sci Date: 2015-10 Impact factor: 4.799