BACKGROUND: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007). OBJECTIVE: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. STUDY DESIGN: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls. RESULTS: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses. CONCLUSION: H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. Copyright (c) 2009 Elsevier B.V. All rights reserved.
BACKGROUND: Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007). OBJECTIVE: To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens. STUDY DESIGN: We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls. RESULTS: The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses. CONCLUSION:H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting. Copyright (c) 2009 Elsevier B.V. All rights reserved.
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Authors: Delia Bethell; David Saunders; Anan Jongkaewwattana; Jarin Kramyu; Arunee Thitithayanont; Suwimon Wiboon-ut; Kosol Yongvanitchit; Amporn Limsalakpetch; Utaiwan Kum-Arb; Nichapat Uthaimongkol; Jean Michel Garcia; Ans E Timmermans; Malik Peiris; Stephen Thomas; Anneke Engering; Richard G Jarman; Duangrat Mongkolsirichaikul; Carl Mason; Nuanpan Khemnu; Stuart D Tyner; Mark M Fukuda; Douglas S Walsh; Sathit Pichyangkul Journal: PLoS One Date: 2013-03-26 Impact factor: 3.240