OBJECTIVE: To compare the contents of iridoid among the fruit, seed, and pericarp from Gardenia jasminoides. METHOD: The contents of geniposide and genipin gentiobioside were determined simultaneously by HPLC. A Kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12: 88) as the mobile phases. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1). RESULT: The obtained linearity of the two components was better over 0.999 4 and the average recoveries were 99.11%, 98.47%, respectively. The results exhibited the remarkable variation among the different parts of the fruit. CONCLUSION: The contents of the two constituents in seed were higher than in pericarp.
OBJECTIVE: To compare the contents of iridoid among the fruit, seed, and pericarp from Gardenia jasminoides. METHOD: The contents of geniposide and genipin gentiobioside were determined simultaneously by HPLC. A Kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12: 88) as the mobile phases. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1). RESULT: The obtained linearity of the two components was better over 0.999 4 and the average recoveries were 99.11%, 98.47%, respectively. The results exhibited the remarkable variation among the different parts of the fruit. CONCLUSION: The contents of the two constituents in seed were higher than in pericarp.