N Blondiaux1, O Gaillot, R-J Courcol. 1. Pôle de Microbiologie, Centre de Biologie Pathologie, Centre Hospitalier Régional Universitaire de Lille, boulevard du Pr-J-Leclercq, 59037 Lille cedex, France. nicolas.blondiaux@chru-lille.fr
Abstract
PURPOSE: The aim of our study was to evaluate the capacity of MALDI-TOF mass spectrometry to identify clinical bacterial isolates, as compared to the automated identification system Vitek 2 (bioMérieux) used routinely in a teaching hospital. METHODS: Three hundred and sixty-two strains representing 178 species from the laboratory collection were analysed by a Microflex spectrometer (Bruker Daltonics) and Vitek 2. Discrepancies between MALDI-TOF and Vitek 2 identifications were investigated by genetic identification (rrS, sodA, rpoB), considered as a reference. RESULTS: Among the 362 isolates, 264 (73%) were consistently identified by Vitek 2 and Microflex. Taking into account genetic identification, we found that 44 (44.9%) of the 98 remaining isolates were correctly identified by mass spectrometry but not by Vitek 2. Conversely, 33 isolates (33.7%) were correctly identified by Vitek 2, but not by Microflex. The genetic identification of the 21 remaining isolates (21,4%) did not match either Vitek 2 or Microflex results. CONCLUSION: The performances of MALDI-TOF mass spectrometry for bacterial identification correspond to those of a reference automated identification system. Copyright (c) 2009 Elsevier Masson SAS. All rights reserved.
PURPOSE: The aim of our study was to evaluate the capacity of MALDI-TOF mass spectrometry to identify clinical bacterial isolates, as compared to the automated identification system Vitek 2 (bioMérieux) used routinely in a teaching hospital. METHODS: Three hundred and sixty-two strains representing 178 species from the laboratory collection were analysed by a Microflex spectrometer (Bruker Daltonics) and Vitek 2. Discrepancies between MALDI-TOF and Vitek 2 identifications were investigated by genetic identification (rrS, sodA, rpoB), considered as a reference. RESULTS: Among the 362 isolates, 264 (73%) were consistently identified by Vitek 2 and Microflex. Taking into account genetic identification, we found that 44 (44.9%) of the 98 remaining isolates were correctly identified by mass spectrometry but not by Vitek 2. Conversely, 33 isolates (33.7%) were correctly identified by Vitek 2, but not by Microflex. The genetic identification of the 21 remaining isolates (21,4%) did not match either Vitek 2 or Microflex results. CONCLUSION: The performances of MALDI-TOF mass spectrometry for bacterial identification correspond to those of a reference automated identification system. Copyright (c) 2009 Elsevier Masson SAS. All rights reserved.
Authors: Laura Ferreira; Fernando Sánchez-Juanes; Magdalena González-Avila; David Cembrero-Fuciños; Ana Herrero-Hernández; José Manuel González-Buitrago; Juan Luis Muñoz-Bellido Journal: J Clin Microbiol Date: 2010-04-14 Impact factor: 5.948
Authors: Christopher Mutuku; Szilvia Melegh; Krisztina Kovacs; Peter Urban; Eszter Virág; Reka Heninger; Robert Herczeg; Ágnes Sonnevend; Attila Gyenesei; Csaba Fekete; Zoltan Gazdag Journal: Antibiotics (Basel) Date: 2022-06-07